============
igv-notebook is a Python package which wraps igv.js for embedding in an IPython notebook. Both Jupyter and Google Colab platforms are supported.
Other projects enabling embedding igv.js in notebooks include
- igv-jupyter (wrapper around igv-notebook that adds g2nb integration)
- igv-jupyterlab
- ipyigv
The main differences between igv-notebook and these other projects are:
- igv-notebook is a Python package, while the projects listed above are Jupyter extensions;
- igv-notebook works with Google Colab, in addition to Jupyter and JupyterLab; and
- igv-notebook supports loading data files from any location on the local or mounted file system when used with Jupyter Notebook or Google Colab. NOTE: General local file paths do not work with JupyterLab, for JupyterLab the files must be in the JupyterLab file tree.
Example notebooks are available in the github repository, and can be run from the Binder and Colab links above. To download examples without cloning the repository use this link. Notebooks are available in the "examples" directory.
Typical usage proceeds as follow
- Install igv-notebook
- Initialize igv-notebook
- Create igv "browser" object
- Add tracks to browser
- Navigate to loci of interest
Installation
pip install igv-notebookInitialization
After installing, import and intialize igv_notebook as follows.
import igv_notebook
igv_notebook.init()For a Jupyter notebook this should be done once per notebook. Colab notebooks display output in a sandboxed iFrame for each cell, so initialization must be repeated for each cell in which igv-notebook is used. `
The Browser initializer takes a configuration dictionary which is converted to JSON and passed to the igv.js createBrowser function. The configuration options are described in the igv.js documentation.
Example:
import igv_notebook
igv_notebook.init()
igv_browser= igv_notebook.Browser(
{
"genome": "hg19",
"locus": "chr22:24,376,166-24,376,456",
"tracks": [{
"name": "BAM",
"url": "https://s3.amazonaws.com/igv.org.demo/gstt1_sample.bam",
"indexURL": "https://s3.amazonaws.com/igv.org.demo/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
}],
"roi": [
{
"name": "ROI set 1",
"url": "https://s3.amazonaws.com/igv.org.test/data/roi/roi_bed_1.bed",
"indexed": False,
"color": "rgba(94,255,1,0.25)"
}
]
}
)Configuration objects for igv.js have properties to specify URLs to files for data and indexes. These properties are
supported in igv-notebook, however igv-notebook also provides equivalent path properties for specfiying paths to
local files when used with Jupyter Notebook or Colab. (Note: The path properties cannot be used with JupyterLab, however local files can
be loaded by URL if they are in the Jupyter file tree). The path properties are useful for:
- loading data in a Colab notebook from the local Colab file system or a mounted Google Drive; and
- loading data in Jupyter Notebook from the local file system that is outside the Jupyter file tree.
URL and Path properties
| igv.js url property | igv-notebook path property |
|---|---|
| url | path |
| indexURL | indexPath |
| fastaURL | fastaPath |
| cytobandURL | cytobandPath |
| aliasURL | aliasPath |
For Jupyter servers (Notebook and Lab), local files can be also be loaded via the url property if the file is in the Jupyter
startup directory tree. This will usually yield better performance than using path properties. URL paths
that begin with a "/" are relative to the Jupyter server startup directory, i.e. the directory from where you
started Jupyter Notebook or JupyterLab. For Jupyter Notebook, URL paths without a leading slash can be used and are
assumed to be relative to the notebook directory. See below for examples. You can also use the "download url" for
the file, obtainable through the JupyterLab UI, as the URL for igv.
To load a track, pass a track configuration object to igv_browser.load_track(). Track configuration
objects are described in the igv.js documentation, however
see the note on URLs and paths above. The configuration object will be converted to JSON and passed to the igv.js browser instance.
Data for the track can be loaded by URL, file path, or passed directly as an array of JSON objects.
Examples:
Local file - Jupyter. URL relative to the location of the notebook
igv_browser.load_track(
{
"name": "Local BAM",
"url": "data/gstt1_sample.bam",
"indexURL": "data/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
})Local file - Jupyter. URL relative to root of Jupyter file tree
igv_browser.load_track(
{
"name": "Local BAM",
"url": "/examples/data/gstt1_sample.bam",
"indexURL": "/examples/data/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
})Local file - Jupyter. Absolute file path, potentially outside the Jupyter file tree. Note the use of path and indexPath.
igv_browser.load_track(
{
"name": "Local BAM",
"path": "/any_path_you_like/data/gstt1_sample.bam",
"indexPath": "/any_path_you_like/data/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
})Local file - Colab. In Colab files are loaded by file path.
igv_browser.load_track(
{
"name": "Local BAM",
"path": "/content/igv-notebook/examples/data/gstt1_sample.bam",
"indexPath": "/content/igv-notebook/examples/data/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
})Remote file - Jupyter.
igv_browser.load_track(
{
"name": "BAM",
"url": "https://s3.amazonaws.com/igv.org.demo/gstt1_sample.bam",
"indexURL": "https://s3.amazonaws.com/igv.org.demo/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
})Most IGV options can be specified in the initial browser configuration, including specifying genome, locus, tracks, and regions-of-interest. Additional methods are provided to perform actions on the browser post-creation. These are described below
To load a track
igv_browser.load_track(track_config)
See example track configurations above. Also igv.js wiki
Example:
igv_browser.load_track(
{
"name": "Local BAM",
"url": "data/gstt1_sample.bam",
"indexURL": "data/gstt1_sample.bam.bai",
"format": "bam",
"type": "alignment"
})Regions-of-interest are overlays marking genomic ranges of interest. They are defined by track configurations, often backed by a "bed" file, and usually with a translucent color. These can be specified at browser creation with the "roi" property, or loaded afterwards with the ```loadROIs`` function. This function takes an array of track configuration objects. See the notebook examples/ROI.ipynb for example usage.
igv_browser.loadROIs([roi_configs])
Jump to a specific genomic range
igv_browser.search('chr1:3000-4000')Jump to a specific gene. This uses the IGV search web service, which currently supports a limited number of genomes. To customize the search, load a non-indexed annotation track with the "searchable" property set to true (see igv.js documentation).
igv_browser.search('myc')Zoom in by a factor of 2
igv_browser.zoom_in()Zoom out by a factor of 2
igv_browser.zoom_out()To convert the current igv view to a static SVG image
igv_browser.to_svg()This action can also be invoked with the "To SVG" button on the igv.js command bar. This is useful when converting the notebook to formats such as HTML and PDF.
Note: This action is not reversible.
To verify the currently installed igv-notebook version (versions >= 0.3.1 only)
igv_notebook.version()To verify the current version of igv.js (igv-notebook versions >= 0.4.0 only)
igv_notebook.igv_version()requires python >= 3.9.1
pip install -e .python setup.py build - Edit VERSION_IGV - enter igv.js version with no line feed. Visit npmjs.com to find latest version
- Run
python updateIGV.py
0.5.2
- Bug fix -- igv undefined
0.5.1
- Update igv.js version to 2.15.7
0.5.0
- Add support for loading an igv session
- Generate links to igv-web
0.4.5
- Fix path/url problems when using JupyterLab
0.4.4
- Add missing requirements to setup.py
- Update igv.js version to 2.13.10
0.4.3
- Fix file-not-found error with version() and igv_version() functions
0.4.1
- Update documentation
0.4.0
- Add support for regions-of-interest
- Add
igv_notebook.igv_version()function.
0.3.1
- Update
browser.to_svg()function to support Python 3.6. - Add
igv_notebook.version()function.
0.3.0
- Add
browser.to_svg()function to convert igv instance to static SVG image (Jupyter Notebook only).
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