Comments (3)
Hi @mvhunter1
This will indeed be the reason of the error and I can and will fix this easily and quickly.
I just have some questions concerning the direct application of NicheNet on 10x spatial transcriptomics data. In many cases, direct application won't be really appropriate I think. So, be sure it's ok to use NicheNet the way you are planning to do it. So this means: you should have changes in gene expression in receiver cells (in the spatial transcriptomics case 'spots containing multiple cells of the same of different cell types) caused by cell-cell interactions.
Feel free to ask me more questions about this if you would be in doubt.
I'll let you know when I fixed this problem.
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Hi @mvhunter1
If you reinstall nichenetr now, you should be able to run the get_expressed_genes()
and seurat wrapper functions on your spatial seurat object
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HI, thank you so much for your quick reply and fix. Yes, in my spatial transcriptomic data I have data from both tumor "spots" and spots from normal tissue surrounding the tumor. I'm looking to use Nichenet to examine communication between these two cell types. Thanks again for your help!
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Related Issues (20)
- NicheNet-v2 data source collection and processing (OmniPath)? HOT 2
- generate_prioritization_tables() generates an empty result. HOT 1
- how to use nichenet in cell development data HOT 4
- there is a warning in this shetp-"score ligand-receptor interactions based on expression strength of the receptor"
- Error on get_prioritization_tables() HOT 1
- Error while running get_expressed_genes HOT 5
- So much targets in nichenet_output$ligand_activity_target_heatmap after running nichenet_seuratobj_aggregate HOT 1
- Modeling Inquiry HOT 2
- Error in "evaluate_target_prediction(setting, ligand_target_matrix, ligands_position) : all genes have same response"
- Is there a helper function to draw the plot in the description ? HOT 1
- Inquiry about Signaling and Gene Regulatory Networks Data in Omnipath and NicheNet HOT 1
- Query: AUPR HOT 1
- Error while plotting a ligand-receptor interaction network
- Error running nichenet_seuratobj_aggregate on v5 Seurat obj HOT 7
- Assistance Needed: nichenetr Package Installation Error in R
- Zenodo Data Inquiry HOT 1
- Identifying bonafide ligand-receptor pairs in the updated ligand-receptor database (V2)
- SeuratV5 HOT 4
- Error finding log fold change information of ligands from sender cells (Seurat V5 object) HOT 6
- How exactly the ligand activity were calculate? HOT 1
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