Tutorial link about nanostringnctools

[NicoleEO]

@amarinderthind, we are working on getting the readme up. If you need some guidance in the meantime here is a link to the vignette: https://github.com/Nanostring-Biostats/NanoStringNCTools/tree/master/vignettes.

[amarinderthind]

Thanks, it seems package requires R 4.1. However, there are a few issues while installing R 4.1 (Pre-release) for the window. Do you have any suggestions?

[zhiiiyang]

@amarinderthind, the description lists the R version to be >=3.6. If you can't install it from Bioconductor due to the lower R version, you can also try devtools::install_github("Nanostring-Biostats/NanoStringNCTools")

https://github.com/Nanostring-Biostats/NanoStringNCTools/blob/master/DESCRIPTION#L10

[amarinderthind]

Hi! Sorry to bother you. I can't find a proper document, particularly how to interpret the geometric mean of the housekeeping plot. If it fails, does it mean hk genes frequently detected below the Limit of detection (LOD)? And in this case, i might be changing the hk genes, or if not, then I should exclude the sample with flags.

[NicoleEO]

Hi @amarinderthind, please refer to our support site for assay questions. Our FAQ page, https://www.nanostring.com/support/documentation/knowledge-base-faqs/#data-analysis-and-normalization-general, may be of interest. Feel free to reach out to our support group if you have additional questions: [email protected].