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Conversion tool/library for converting whole slide images to DICOM

License: Apache License 2.0

CMake 0.83% Shell 5.27% C 0.82% C++ 92.61% Ruby 0.29% Python 0.18%
dicom wsi openslide

wsi-to-dicom-converter's Introduction

WSI to DICOM Converter

This repository contains a tool that converts whole slide images (WSIs) to DICOM. To read the underlying whole slide images (WSIs), this tool relies on OpenSlide, which supports a variety of file formats.

Quickstart

Debian-based Linux

To download the latest release, on the Releases tab, download the installer for your operating system and then run it. For example, if you're running on a Debian-based system, download the wsi2dcm_x.y.z.deb file and then run:

sudo apt install ./wsi2dcm_x.y.z.deb

Note: if you get an error about missing shared libraries, run sudo ldconfig or make sure that /usr/local/lib is in your LD_LIBRARY_PATH.

You may also need to install the following packages and their dependencies with sudo apt-get install:

  • libtiff-dev
  • libxml2-dev
  • libcairo-dev
  • gtk2-engines-pixbuf

MacOS

brew install wsi2dcm.rb

If an installer isn't available for your operating system, see Compiling from source.

After you have installed the WSI to DICOM converter, to convert a file, run the following command:

wsi2dcm --input <wsiFile> --outFolder <folder for generated files> --seriesDescription <text description>

Test data is freely available from OpenSlide.

To see all available options, run: wsi2dcm --help.

Complete set of parameters

input

Input wsi file, supported by openslide.

outFolder

Folder to store dcm files

tileHeight

Tile height px.

tileWidth

Tile width px.

levels

Number of levels to generate, levels == 0 means number of levels will be read from wsi file.

downsamples

Size factor for each level for each level, downsample is size factor for each level.

eg: if base level size is 100x100 and downsamples is (1, 2, 10) then

  • level0 100x100
  • level1 50x50
  • level2 10x10
startOn

Level to start generation.

stopOn

Level to stop generation.

sparse

Use TILED_SPARSE frame organization, by default it's TILED_FULL http://dicom.nema.org/medical/dicom/current/output/chtml/part03/sect_C.7.6.17.3.html

compression

Compression, supported compressions: jpeg, jpeg2000, raw.

seriesDescription

(0008,103E) [LO] SeriesDescription Dicom tag.

studyId

(0020,000D) [UI] StudyInstanceUID Dicom tag.

seriesId

(0020,000E) [UI] SeriesInstanceUID Dicom tag.

jsonFile

Dicom json file with additional tags https://www.dicomstandard.org/dicomweb/dicom-json-format/

batch

Maximum frames in one file, as limit is exceeded new files is started.

eg: 3 files will be generated if batch is 10 and 30 frames in level

threads

Threads to consume during execution.

debug

Print debug messages: dimensions of levels, size of frames.

dropFirstRowAndColumn

Drop first row and column of the source image in order to workaround bug openslide/openslide#268

Compiling from source

If you're using Ubuntu, run the following command to download the dependencies and build the tool:

sudo ./cloud_build/ubuntuBuild.sh

Otherwise, follow these steps:

  1. Download the source from the Releases tab or check out the repo.
  2. Make sure that you have the following dependencies installed:
  1. Run the following commands:
mkdir build
cd build
cp -R %dcmtkDir% ./dcmtk-3.6.6
cmake ..
make -j%threads%

wsi-to-dicom-converter's People

Contributors

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wsi-to-dicom-converter's Issues

build fails with boost >= 1.71

Boost seem to have changed something in their cmake files and this has broken CMake/Boost.cmake. Running cmake generates errors like:

$ cmake ..
CMake Error at /usr/lib/x86_64-linux-gnu/cmake/Boost-1.74.0/BoostConfig.cmake:258 (boost_find_component):
  boost_find_component Macro invoked with incorrect arguments for macro
  named: boost_find_component
Call Stack (most recent call first):
  /usr/share/cmake-3.18/Modules/FindBoost.cmake:448 (find_package)
  CMake/Boost.cmake:25 (find_package)
  CMakeLists.txt:29 (include)

... etc.

1.71 has the same issue.

Adhere to Google Style Guide

Code must adhere to https://google.github.io/styleguide/cppguide.html.

Few examples:

DCM output images showing up black on some levels

  1. Using code from develop (282325)
  2. Building and running within a vanilla ubuntu docker image
  3. All dependency installed and built via ./cloud_build/ubuntuBuild.sh
  4. Using CMU-2.svs as input data
  5. example command: wsi2dcm --input CMU-2.svs --outFolder cmuout/ --startOn 4 --levels 8 --debug
    output:
[2022-03-14 18:46:57.164381] [0x0000004008394340] [info]    dicomization is started
[2022-03-14 18:46:57.165676] [0x0000004008394340] [warning] threads parameter is less than 1, consuming all avalible threads
[2022-03-14 18:46:57.165774] [0x0000004008394340] [warning] batch parameter is not set, batch is unlimited
[2022-03-14 18:46:57.501650] [0x0000004008394340] [debug]    
[2022-03-14 18:46:57.502081] [0x0000004008394340] [debug]   Level Count: 4
[2022-03-14 18:46:57.503398] [0x0000004008394340] [debug]   Uncropped dimensions Level[4]: 4876, 1903
[2022-03-14 18:46:57.503484] [0x0000004008394340] [debug]   Set Smallest
[2022-03-14 18:46:57.503726] [0x0000004008394340] [debug]   Level[4] frames:10, 4
[2022-03-14 18:46:57.503796] [0x0000004008394340] [debug]   Uncropped dimensions Level[5]: 2438, 951
[2022-03-14 18:46:57.503885] [0x0000004008394340] [debug]   Set Smallest
[2022-03-14 18:46:57.503945] [0x0000004008394340] [debug]   Level[5] frames:5, 2
[2022-03-14 18:46:57.504365] [0x0000004008394340] [debug]   Uncropped dimensions Level[6]: 1219, 475
[2022-03-14 18:46:57.504399] [0x0000004008394340] [debug]   Set Smallest
[2022-03-14 18:46:57.504474] [0x0000004008394340] [debug]   Level[6] frames:3, 1
[2022-03-14 18:46:57.504633] [0x0000004008394340] [debug]   Uncropped dimensions Level[7]: 609, 237
[2022-03-14 18:46:57.504653] [0x0000004008394340] [debug]   Set Smallest
[2022-03-14 18:46:57.504721] [0x0000004008394340] [debug]   Level[7] frames:2, 1
[2022-03-14 18:46:57.504774] [0x0000004008394340] [debug]   Uncropped dimensions Level[8]: 304, 118
[2022-03-14 18:46:57.504827] [0x0000004008394340] [debug]   Set Smallest
[2022-03-14 18:46:57.504998] [0x0000004008394340] [debug]   Level[8] frames:1, 1
[2022-03-14 18:46:57.791183] [0x0000004008394340] [debug]    
[2022-03-14 18:46:57.791307] [0x0000004008394340] [debug]   Starting Level 4
[2022-03-14 18:46:57.791860] [0x0000004008394340] [debug]   level size: 4875 1903 16.0037
[2022-03-14 18:46:57.791957] [0x0000004008394340] [debug]   multiplicator: 16.0037
[2022-03-14 18:46:57.792029] [0x0000004008394340] [debug]   levelToGet: 2
[2022-03-14 18:46:57.792097] [0x0000004008394340] [debug]   downsample: 16
[2022-03-14 18:46:57.792173] [0x0000004008394340] [debug]   downsampleOfLevel: 0.99977
[2022-03-14 18:46:57.792245] [0x0000004008394340] [debug]   frameDownsampled: 499, 499
[2022-03-14 18:46:57.792384] [0x0000004008394340] [debug]   higherMagnifcationDicomFiles 0
[2022-03-14 18:46:57.793147] [0x0000004008394340] [debug]   Level Frame Count: 40
[2022-03-14 18:46:58.430072] [0x00000040094b1700] [debug]    frame size: 11kb
[2022-03-14 18:46:58.448414] [0x0000004008cb0700] [debug]    frame size: 16kb
[2022-03-14 18:46:58.826128] [0x000000400acb4700] [debug]    frame size: 4kb
[2022-03-14 18:46:58.873890] [0x000000400bcb6700] [debug]    frame size: 4kb
[2022-03-14 18:46:58.915102] [0x000000400b4b5700] [debug]    frame size: 4kb
[2022-03-14 18:46:58.923300] [0x0000004009cb2700] [debug]    frame size: 4kb
[2022-03-14 18:46:58.930410] [0x000000400c4b7700] [debug]    frame size: 4kb
[2022-03-14 18:46:59.058290] [0x000000400a4b3700] [debug]    frame size: 4kb
[2022-03-14 18:46:59.332207] [0x00000040094b1700] [debug]    frame size: 4kb
[2022-03-14 18:46:59.504926] [0x0000004008cb0700] [debug]    frame size: 4kb
[2022-03-14 18:46:59.635306] [0x000000400acb4700] [debug]    frame size: 7kb
[2022-03-14 18:46:59.869747] [0x000000400b4b5700] [debug]    frame size: 71kb
[2022-03-14 18:46:59.915873] [0x000000400bcb6700] [debug]    frame size: 70kb
[2022-03-14 18:46:59.951229] [0x0000004009cb2700] [debug]    frame size: 51kb
[2022-03-14 18:47:00.057248] [0x000000400c4b7700] [debug]    frame size: 5kb
[2022-03-14 18:47:00.156447] [0x000000400a4b3700] [debug]    frame size: 5kb
[2022-03-14 18:47:00.353829] [0x00000040094b1700] [debug]    frame size: 66kb
[2022-03-14 18:47:00.541982] [0x000000400acb4700] [debug]    frame size: 55kb
[2022-03-14 18:47:00.605109] [0x0000004008cb0700] [debug]    frame size: 73kb
[2022-03-14 18:47:00.900731] [0x000000400b4b5700] [debug]    frame size: 9kb
[2022-03-14 18:47:00.903478] [0x000000400bcb6700] [debug]    frame size: 7kb
[2022-03-14 18:47:01.020370] [0x0000004009cb2700] [debug]    frame size: 73kb
[2022-03-14 18:47:01.081352] [0x000000400a4b3700] [debug]    frame size: 49kb
[2022-03-14 18:47:01.235981] [0x000000400c4b7700] [debug]    frame size: 83kb
[2022-03-14 18:47:01.278671] [0x00000040094b1700] [debug]    frame size: 5kb
[2022-03-14 18:47:01.528315] [0x000000400acb4700] [debug]    frame size: 5kb
[2022-03-14 18:47:01.788026] [0x0000004008cb0700] [debug]    frame size: 51kb
[2022-03-14 18:47:02.098192] [0x000000400b4b5700] [debug]    frame size: 84kb
[2022-03-14 18:47:02.100359] [0x000000400bcb6700] [debug]    frame size: 88kb
[2022-03-14 18:47:02.206806] [0x000000400a4b3700] [debug]    frame size: 7kb
[2022-03-14 18:47:02.272262] [0x0000004009cb2700] [debug]    frame size: 14kb
[2022-03-14 18:47:02.314133] [0x00000040094b1700] [debug]    frame size: 10kb
[2022-03-14 18:47:02.542332] [0x000000400c4b7700] [debug]    frame size: 10kb
[2022-03-14 18:47:02.651156] [0x000000400acb4700] [debug]    frame size: 5kb
[2022-03-14 18:47:02.776733] [0x0000004008cb0700] [debug]    frame size: 5kb
[2022-03-14 18:47:02.999762] [0x000000400bcb6700] [debug]    frame size: 16kb
[2022-03-14 18:47:03.022870] [0x000000400b4b5700] [debug]    frame size: 5kb
[2022-03-14 18:47:03.082044] [0x0000004009cb2700] [debug]    frame size: 20kb
[2022-03-14 18:47:03.090988] [0x000000400a4b3700] [debug]    frame size: 40kb
[2022-03-14 18:47:03.142935] [0x00000040094b1700] [debug]    frame size: 6kb
[2022-03-14 18:47:03.572745] [0x0000004008394340] [debug]    
[2022-03-14 18:47:03.572991] [0x0000004008394340] [debug]   Starting Level 5
[2022-03-14 18:47:03.573448] [0x0000004008394340] [debug]   level size: 2437 951 32.0191
[2022-03-14 18:47:03.573786] [0x0000004008394340] [debug]   multiplicator: 32.0191
[2022-03-14 18:47:03.573875] [0x0000004008394340] [debug]   levelToGet: 3
[2022-03-14 18:47:03.573953] [0x0000004008394340] [debug]   downsample: 32
[2022-03-14 18:47:03.574027] [0x0000004008394340] [debug]   downsampleOfLevel: 0.999405
[2022-03-14 18:47:03.574149] [0x0000004008394340] [debug]   frameDownsampled: 499, 499
[2022-03-14 18:47:03.574300] [0x0000004008394340] [debug]   higherMagnifcationDicomFiles 0
[2022-03-14 18:47:03.574698] [0x0000004008394340] [debug]   Level Frame Count: 10
[2022-03-14 18:47:04.099722] [0x0000004008cb0700] [debug]    frame size: 40kb
[2022-03-14 18:47:04.224257] [0x000000404ede4700] [debug]    frame size: 24kb
[2022-03-14 18:47:04.246746] [0x000000404e5e3700] [debug]    frame size: 6kb
[2022-03-14 18:47:04.590061] [0x000000400a4b3700] [debug]    frame size: 7kb
[2022-03-14 18:47:04.601154] [0x0000004009cb2700] [debug]    frame size: 4kb
[2022-03-14 18:47:04.620269] [0x00000040094b1700] [debug]    frame size: 4kb
[2022-03-14 18:47:04.642179] [0x000000404f5e5700] [debug]    frame size: 6kb
[2022-03-14 18:47:04.673208] [0x000000404dde2700] [debug]    frame size: 36kb
[2022-03-14 18:47:04.875972] [0x0000004008cb0700] [debug]    frame size: 46kb
[2022-03-14 18:47:04.931069] [0x000000404ede4700] [debug]    frame size: 33kb
[2022-03-14 18:47:05.315167] [0x0000004008394340] [debug]    
[2022-03-14 18:47:05.315233] [0x0000004008394340] [debug]   Starting Level 6
[2022-03-14 18:47:05.315273] [0x0000004008394340] [debug]   level size: 2437 951 32.0191
[2022-03-14 18:47:05.315301] [0x0000004008394340] [debug]   multiplicator: 32.0191
[2022-03-14 18:47:05.315320] [0x0000004008394340] [debug]   levelToGet: 3
[2022-03-14 18:47:05.315337] [0x0000004008394340] [debug]   downsample: 64
[2022-03-14 18:47:05.315359] [0x0000004008394340] [debug]   downsampleOfLevel: 1.99881
[2022-03-14 18:47:05.315379] [0x0000004008394340] [debug]   frameDownsampled: 999, 951
[2022-03-14 18:47:05.315398] [0x0000004008394340] [debug]   higherMagnifcationDicomFiles 0
[2022-03-14 18:47:05.315432] [0x0000004008394340] [debug]   Level Frame Count: 3
[2022-03-14 18:47:06.244938] [0x000000400a4b3700] [debug]    frame size: 32kb
[2022-03-14 18:47:06.252177] [0x00000040094b1700] [debug]    frame size: 47kb
[2022-03-14 18:47:06.477401] [0x00000040505e7700] [debug]    frame size: 4kb
[2022-03-14 18:47:06.828315] [0x0000004008394340] [debug]    
[2022-03-14 18:47:06.828386] [0x0000004008394340] [debug]   Starting Level 7
[2022-03-14 18:47:06.828429] [0x0000004008394340] [debug]   level size: 2437 951 32.0191
[2022-03-14 18:47:06.828468] [0x0000004008394340] [debug]   multiplicator: 32.0191
[2022-03-14 18:47:06.828615] [0x0000004008394340] [debug]   levelToGet: 3
[2022-03-14 18:47:06.828704] [0x0000004008394340] [debug]   downsample: 128
[2022-03-14 18:47:06.828744] [0x0000004008394340] [debug]   downsampleOfLevel: 3.99762
[2022-03-14 18:47:06.828768] [0x0000004008394340] [debug]   frameDownsampled: 1998, 951
[2022-03-14 18:47:06.828801] [0x0000004008394340] [debug]   higherMagnifcationDicomFiles 0
[2022-03-14 18:47:06.828845] [0x0000004008394340] [debug]   Level Frame Count: 2
[2022-03-14 18:47:07.908224] [0x000000400a4b3700] [debug]    frame size: 2kb
[2022-03-14 18:47:08.392908] [0x00000040533aa700] [debug]    frame size: 23kb
[2022-03-14 18:47:08.787137] [0x0000004008394340] [debug]    
[2022-03-14 18:47:08.787239] [0x0000004008394340] [debug]   Starting Level 8
[2022-03-14 18:47:08.787302] [0x0000004008394340] [debug]   level size: 2437 951 32.0191
[2022-03-14 18:47:08.787341] [0x0000004008394340] [debug]   multiplicator: 32.0191
[2022-03-14 18:47:08.787367] [0x0000004008394340] [debug]   levelToGet: 3
[2022-03-14 18:47:08.787390] [0x0000004008394340] [debug]   downsample: 256
[2022-03-14 18:47:08.787426] [0x0000004008394340] [debug]   downsampleOfLevel: 7.99524
[2022-03-14 18:47:08.787457] [0x0000004008394340] [debug]   frameDownsampled: 2437, 951
[2022-03-14 18:47:08.787479] [0x0000004008394340] [debug]   higherMagnifcationDicomFiles 0
[2022-03-14 18:47:08.787720] [0x0000004008394340] [debug]   Level Frame Count: 1
[2022-03-14 18:47:10.571696] [0x00000040588b1700] [debug]    frame size: 8kb
[2022-03-14 18:47:10.645810] [0x0000004008394340] [info]    dicomization is done

I initially loaded these into GCP's DICOM web store and noticed that the OHIF viewer was showing some images as empty. I confirmed the similar odd behavior locally with QuPath. Note the black/corrupt previews in QuPath:

image

Let me know if there is any other information I can provide

Compiling fails on MacOS with failure to find jpeg_mem_dest, was failing finding openjpeg 2.3.1

[ 96%] Building CXX object CMakeFiles/wsi2dcm.dir/src/jpeg2000Compression.cpp.o
/Users/dclunie/Work/wsi2dcm/wsi-to-dicom-converter-1.0/src/jpeg2000Compression.cpp:80:3: error: no matching function for call to 'opj_stream_set_write_function'
opj_stream_set_write_function(
^~~~~~~~~~~~~~~~~~~~~~~~~~~~~
/opt/local/include/openjpeg-2.3/openjpeg.h:1169:27: note: candidate function not viable: no known conversion from '(lambda at /Users/dclunie/Work/wsi2dcm/wsi-to-dicom-converter-1.0/src/jpeg2000Compression.cpp:81:12)' to 'opj_stream_write_fn'
(aka 'unsigned long (*)(void *, unsigned long, void )') for 2nd argument
OPJ_API void OPJ_CALLCONV opj_stream_set_write_function(opj_stream_t
p_stream,
^
1 error generated.
make[2]: *** [CMakeFiles/wsi2dcm.dir/src/jpeg2000Compression.cpp.o] Error 1

This is with openjpeg 2.3.1 installed through macports.

David

libjsoncpp 1.9.x: use of deleted function ‘Json::Value::Value(std::nullptr_t)’

For some reason I cannot compile latest wsi2dcm on my Debian machine:

wsi-to-dicom-converter/src/dcmTags.cpp:28:26: error: use of deleted function ‘Json::Value::Value(std::nullptr_t)’
   28 |   if (obj[tag][VALUE] != nullptr) {
      |                          ^~~~~~~
In file included from /usr/include/jsoncpp/json/reader.h:11,
                 from /usr/include/jsoncpp/json/json.h:11,
                 from wsi-to-dicom-converter/src/dcmTags.cpp:19:
/usr/include/jsoncpp/json/value.h:345:3: note: declared here
  345 |   Value(std::nullptr_t ptr) = delete;
      |   ^~~~~

With:

% apt-cache policy libjsoncpp-dev
libjsoncpp-dev:
  Installed: 1.9.4-4
  Candidate: 1.9.4-4
  Version table:
 *** 1.9.4-4 500
        500 http://deb.debian.org/debian bullseye/main amd64 Packages
        100 /var/lib/dpkg/status

Add end-to-end test

On top of unit tests that validate smaller chunks of behavior there should be an end-to-end test that runs the CLI tool on some input files and validates the output is as expected.

Blacked out Information

Hi all-
Thanks for this converter. I am using this tool in conjunction with QuPath m2 and Google Cloud Platform's datastores. I've noticed some of my .SVS images when converting to dicom format have blacked out squares. The images look complete when viewing as .svs, but then are missing information post conversion. I've converted both through command line, and also loading into qupath and synchronizing to google cloud platform (which I believe uses this tool in the background as well). I was wondering if you had any insight on this or any work arounds?

wsi converter v1.0.2
Qupath -0.2.0-m2
qupath-chcapi-extension v2.0.2
mac 10.13.6

Thanks!
Nick

Installing .deb package requires running ldconfig afterwards

After running sudo apt install ./wsi2dcm_0.1.1.deb all succeeds, but when I try and run the tool I get

wsi2dcm: error while loading shared libraries: libwsi2dcm.so: cannot open shared object file: No such file or directory

Running sudo ldconfig fixes this though.

Can we run ldconfig automatically as part of the postinst script?

d

.

how can I make OUTPUT to be a single dicom file with multiple levels?

First of all, THANK YOU!, for creating this awesome stuff.

Hi, I am trying to build a web application, a dicom file viewer, for personal project.

I need to convert all my svs files to dicom files.
And it seems like outputs are always N number of files, being equivalent to number of levels.

so how can I make OUTPUT to be a single dicom file with multiple levels?

it d be nice if I can get at least hint from you guys... :((((

thank you in advance~!

cheers, from Seoul

Image Center Point Coordinates Sequence

Hi, first thank you for the work this is a really good conversion tool.

Second this is more of a feature request is it possible to calculate the X and Y Offsets (0040,072A) ,(0040,073A) ?

I am using the resulting dicom with a slide microscopy plugin for ohif dicom viewer which needs these values for calculating the overlays when drawing points of interest.

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