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twobitreader's Issues

Invalid nucleotides for last chromosome

Using the following reference genome:

>FIRST_CHR
ACGTACGTACGTACGTACGTACGTACGTA
>LAST_CHR
AAAGGGGGGC

The entire sequence of "LAST_CHR" is correct:

>>> reader['LAST_CHR'][0:9]
'AAAGGGGGG'

But extracting nucleotides from the end of "LAST_CHR" returns wrong nucleotides:

>>> reader = twobitreader.TwoBitFile("genome.2bit")
>>> reader['LAST_CHR'][5:6]
'A'
>>> reader['LAST_CHR'][6:9]
'AAA'

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