yannikschaelte / scvelo

RNA Velocity generalized through dynamical modeling

scVelo - RNA velocity generalized through dynamical modeling

scVelo is a scalable toolkit for RNA velocity analysis in single cells.
The methods are based on Bergen et al. (Nature Biotech, 2020).

RNA velocity enables the recovery of directed dynamic information by leveraging splicing information. scVelo generalizes the concept of RNA velocity (La Manno et al., Nature, 2018) by relaxing previously made assumptions with a stochastic and a dynamical model that solves the full transcriptional dynamics. It thereby adapts RNA velocity to widely varying specifications such as non-stationary populations.

scVelo is compatible with scanpy and hosts efficient implementations of all RNA velocity models.

See https://scvelo.org for documentation and tutorials.

scVelo's key applications

• estimate RNA velocity to study cellular dynamics.
• identify putative driver genes and regimes of regulatory changes.
• infer a latent time to reconstruct the temporal sequence of transcriptomic events.
• estimate reaction rates of transcription, splicing and degradation.
• use statistical tests, e.g., to detect different kinetics regimes.

Upcoming talks

• Sep 15: Temporal Single-Cell Analysis (SCOG)
• Nov 12: Single Cell Biology (SCB)

Reference

Bergen et al. (2020), Generalizing RNA velocity to transient cell states through dynamical modeling, Nature Biotechnology.

Support

Feel free to submit an issue or send us an email. Your help to improve scVelo is highly appreciated. As GitHub requests currently exceeds our limited time capacities, please excuse potential delays. For urgent matters, you may schedule a zoom meeting here.