"No data uploaded" message with custom input about amica

[tbaccata]

Glad it works! I'll add more informative error messages.

Best,
Sebastian

[tbaccata]

Dear Silas,

does it work when all tab-separated files (design, contrasts, specification file) file extensions end with ".txt" (or .tsv, or .dat)?
I could reproduce the error message, when one of this files doesn't have an appropriate file extension.

The file extension for custom proteingroups file shouldn't matter.

Best,
Sebastian

[silasmellor]

Thanks Sebastian,

All the files were saved from excel as .txt (tab separated). I tried both .txt and .tsv. I managed to get it to work by changing the column headers to use those from the example data. I only have proteinID, geneName and LFQ columns (as i understand it the DIA-NN output is already filtered to exclude one hit wonders so i left that out of the analysis). I am honestly not sure why the specifications file i originally supplied was not acceptable, but i suspect the issue is a mismatch somewhere in the columne headers and/or specification.