shengyongniu / rseqtu Goto Github PK

After installing all dependencies I try to install the rSeqTU package from this repsitory and I get:
* installing source package 'rSeqTU' ...
** using staged installation
** R
** byte-compile and prepare package for lazy loading
Warning: replacing previous import 'caret::dotPlot' by 'seqinr::dotPlot' when loading 'rSeqTU'
Warning: replacing previous import 'plyr::count' by 'seqinr::count' when loading 'rSeqTU'
** help
*** installing help indices
converting help for package 'rSeqTU'
finding HTML links ... done
TU_SVM html
gen_NA html
gen_cTU_data html
generate_GTF_training html
** building package indices
** testing if installed package can be loaded from temporary location
*** arch - i386
Error: package or namespace load failed for 'rSeqTU' in library.dynam(lib, package, package.lib):
DLL 'QuasR' not found: maybe not installed for this architecture?
Error: loading failed
Execution halted
*** arch - x64
Warning: replacing previous import 'caret::dotPlot' by 'seqinr::dotPlot' when loading 'rSeqTU'
Warning: replacing previous import 'plyr::count' by 'seqinr::count' when loading 'rSeqTU'
ERROR: loading failed for 'i386'
* removing 'C:/Users/milenovic/Documents/R/win-library/4.1/rSeqTU'
Warning in install.packages :
installation of package ‘C:/Users/milenovic/Downloads/rSeqTU_0.1.0.tar.gz’ had non-zero exit status`

When I try to just load the QuasR package using library(QuasR) that works, but I get the same error when I try to install rSeqTU.
Any ideas?

Is it possible to use this tool on draft genome assemblies? I am trying to run an analysis by mapping reads to an incomplete genome but am running into errors on the "gen_cTU_data" step and was wondering if I had to modify my files in any way.

After installing rseqTU, an error occurred when running qAlign. The error is as follows:

> proj <- qAlign(sampleFile, genomeFile, paired="fr", clObj = makeCluster(detectCores()))
alignment files missing - need to:
    create 3 genomic alignment(s)
will start in ..9s..8s..7s..6s..5s..4s..3s..2s..1s
Testing the compute nodes...OK
Loading QuasR on the compute nodes...OK
Available cores:
xxx: 24
Performing genomic alignments for 3 samples. See progress in the log file:
./QuasR_log_2cfee465e3c17.txt
Error in checkForRemoteErrors(val) : 
  one node produced an error: Error on xxx processing sample /tmp/RtmpWrupMt/WT-1-1_filtered_1P.fq.gz2d015358f2bd1.fastq : Cluster setup failed. 24 of 24 workers failed to connect.