Comments (2)
Hi teyden
I can understand why you get confused. What I mean by absolute abundances are actual absolute abundances. They are not technically impossible if you have external data, such as qPCR or flow cytometry counts. To me it is confusing to call the raw counts absolute abundances, because they are still relative, even though not being normalised or scaled.
So when relative=TRUE DAtest is normalizing/scaling data internally, so raw counts are expected. When relative=FALSE there is no normalization/scaling internally, so absolute abundances, in the true sense, or pre-normalized data is expected.
I hope it makes sense.
Cheers,
Jakob
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