Comments (20)
I just tried again with command: ./genefuse -r ~/data/ref/hg19.fa -1 ~/data/fq/genefuse.R1.fq.gz -2 ~/data/fq/genefuse.R2.fq.gz -h test.html -j test.json -f genes/druggable.hg19.csv
and got:
15:1:4 start with 4 threads
15:1:47 mapper indexing done
15:2:38 sequence number before filtering: 1329
15:2:38 removeByComplexity: 0
15:2:38 removeByDistance: 39
15:2:38 removeIndels: 67
15:4:3 matcher indexing done
15:4:3 removeAlignables: 8
Probably you used incorrect reference? I used hg19 downloaded from UCSC. Did you checked the downloaded files using MD5?
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Hi sfchen,
I have the same problem. I used all the demo files you provided including the reference genome. but I still got nothing in my result.
from genefuse.
Hi sfchen,
I have experienced the same problem as others have reported here. Have you gotten to the bottom of this?
Thanks.
from genefuse.
Can you guys check md5 for the downloaded FASTQ file?
from genefuse.
http://opengene.org/dataset.html
You should download following files:
Paired-end FASTQ files for GeneFuse testing (Illumina platform)
genefuse.R1.fq.gz (size: 62 M, MD5: 171e6dfa0af37fe95c826005bc5fcdf9)
genefuse.R2.fq.gz (size: 66 M, MD5: e756cf01e256186dccaa9e700d85a342)
from genefuse.
Hi sfchen,
Yes those are the same md5 I get when I check on the downloaded FASTQs. The command I run is: ./genefuse -r Homo_sapiens_assembly19.fasta -f druggable.hg19.csv -1 genefuse.R1.fq.gz -2 genefuse.R2.fq.gz -h report.html >result
I have downloaded the .fasta file from ensembl.
Thanks.
from genefuse.
The druggable.hg19.csv is in the genes
folder
Have you checked the error message?
from genefuse.
I mean, you should run:
./genefuse -r Homo_sapiens_assembly19.fasta -f genes/druggable.hg19.csv -1 genefuse.R1.fq.gz -2 genefuse.R2.fq.gz -h report.html >result
from genefuse.
I have downloaded via wget the druggable.hg.csv from the genes folder. In the results document there are no reported errors
from genefuse.
Errors are saved to STDERR, not STDOUT. So you cannot find errors in the result file.
Can you just run the command without redirecting to result?
from genefuse.
I do not get any STDERR or STDOUT files regardless of if I redirect to result or not. I am running the binary if this may make a difference. Thank you for your help by the way.
from genefuse.
Apologies I meant I do not get an STDOUT file at all.
from genefuse.
You used >
, which redirected STDOUT to the file you specified.
from genefuse.
But even if I exclude > there is no STDERR file - that is what I meant not the lack of STDOUT apologies for confusion.
from genefuse.
You didn't redirect STDERR, so it would be printed on terminal.
You can use following command to also redirect STDERR:
./genefuse -r Homo_sapiens_assembly19.fasta -f druggable.hg19.csv -1 genefuse.R1.fq.gz -2 genefuse.R2.fq.gz -h report.html >result & 2>err.log
from genefuse.
I have ran again and no errors are reported. I notice however the FASTQ files don't have the 15 million lines you mention in a different thread - they have more like 800,000. Maybe this is the issue?
from genefuse.
Hi sfchen, I have again ran genefuse on fastqs which I know to contain translocations. Your software did not call these. This is more just to let you know than a specific request or question.
from genefuse.
Thanks, I will try to reproduce it.
from genefuse.
Hi sfchen,
I am in the same situation. I do not find genefusion in the test dataset.
from genefuse.
I found the answer in #31 -- the NCBI version of hg19 had a different chromosome naming convention, so it doesn't work. The version downloadable from UCSC is fine:
wget http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/hg19.fa.gz
(then unzip)
from genefuse.
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