Comments (3)
POS/NEG are used to normalise the data at a more technical level. This includes housekeeping genes.
Housekeeping genes are used (when available through NanoString panel or "prediction") to normalise the data at a "biological level".
from nacho.
Hi Mcanouil, this means, that the POS/NEG labels would not influence the predicted housekeeping genes?
from nacho.
POS/NEG are technical controls with known concentrations of biological materials, thus has nothing to do with your biological samples and/or housekeeping genes.
Their sole purpose is to be used to correct technical bias that might arise.
from nacho.
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from nacho.