The aim of this small project was to check if there was any difference in filtered solutions using different syringe filters.
Different solvents (water, acetonitrile, methanol and isopropanol) were filtered using 3 different types of syringe filters (Millipore, Clarify and Minisart).
Filtered solvents were injected in full scan mode using both negative and positive ionization modes.
This repo contains the codes used for process the adquired data.
- Convert .raw files to .mzXML files with Proteowizard.
- Apply the code remove_orbitrap_shoulder_peak to remove background signals.
- Apply the code BPC to plot the base peak chromatogram according to each type of samples.
- Apply code XCMS_processing to process the data and get the main features.
- Apply the code PCA to manually integrate the detected peaks and get an overview through a PCA.