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exprset's Introduction

exprSet 一个表达矩阵处理工具

输入数据

一、表达矩阵

SRR1039508 SRR1039509 SRR1039512 SRR1039513 SRR1039516 SRR1039517 SRR1039520 SRR1039521
ENSG00000000003 679 448 873 408 1138 1047 770 572
ENSG00000000005 0 0 0 0 0 0 0 0
ENSG00000000419 467 515 621 365 587 799 417 508
ENSG00000000457 260 211 263 164 245 331 233 229
ENSG00000000460 60 55 40 35 78 63 76 60
ENSG00000000938 0 0 2 0 1 0 0 0
ENSG00000000971 3251 3679 6177 4252 6721 11027 5176 7995
ENSG00000001036 1433 1062 1733 881 1424 1439 1359 1109
ENSG00000001084 519 380 595 493 820 714 696 704
ENSG00000001167 394 236 464 175 658 584 360 269
ENSG00000001460 172 168 264 118 241 210 155 177
ENSG00000001461 2112 1867 5137 2657 2735 2751 2467 2905
ENSG00000001497 524 488 638 357 676 806 493 475
ENSG00000001561 71 51 211 156 23 38 134 172

第一列是基因ID,后面的列是各个样本。其中第一行尤为注意,最开头是一个空格(了解R里面read.table函数原理)。

可以看到上面的测试数据是7个样本的表达矩阵,而且来自于转录组测序数据的counts结果,所以表达量都是整数。

二、分组信息

sampleID group
SRR1039508 untrt
SRR1039509 trt
SRR1039512 untrt
SRR1039513 trt
SRR1039516 untrt
SRR1039517 trt
SRR1039520 untrt
SRR1039521 trt

只需要两列即可,其中第一列需要与上面的表达矩阵的样本名一一对应清楚。

三、基因注释信息

ensembl type symbol
ENSG00000000003 protein_coding TSPAN6
ENSG00000000005 protein_coding TNMD
ENSG00000000419 protein_coding DPM1
ENSG00000000457 protein_coding SCYL3
ENSG00000000460 protein_coding C1orf112
ENSG00000000938 protein_coding FGR
ENSG00000000971 protein_coding CFH
ENSG00000001036 protein_coding FUCA2
ENSG00000001084 protein_coding GCLC

多少列无所谓,只需要第一列跟表达矩阵的列名一一对应即可。

界面一:上传并且探索输入数据

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