The scripts includes the following steps: 1 genes that can be binarized are filtered. 2 RNA velocity analysis with Dynamo: RNA velocity and transition graph 3 simulate trajectory on the transition graph 4 calcualtion of reaction coordinates(RCs) 5 Gene regulation network (GRN) inference with partial least square reguration 6 local false discover rate. Then the frustration score along the RCs 7 Gene regulation network (GRN) inference with GRISLI and frustration score calculation 8 GRN analysis:community detection
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