hisashin / dninja

Opensource Digital PCR

dNinja, world's first open-source digital PCR

We have been making open-source PCR machines for decade. 1st generation thermal cycler NinjaPCR was released in 2013. Super fast PCR machines (video1, video2) in 2016 and 2017. During Covid pandemic 2020-2023, we developed $220 qNinja: 2nd generation qPCR (RT-PCR) and $45 qLAMP. Now let us start 3rd generation dNinja, Digital PCR (dPCR). dPCR is 1,000 times more sensitive than qPCR and essential to liquid biopsy for noncomunicable diseases that caused 74% of global death in 2019. Check wiki for details.

Fundraising

We are fundraising at experiment.com.

Stencil Data Generator

dNinja Stencil Generator

You can run this generator inside your own PC once you installed Docker by this command.

docker run --name dninja-stencil -p 8080:8080 -d hisashin/dninja-stencil:latest

Then open http://localhost:8080 in any browser.

• Dockerfile
• Docker Hub
• 142x142 holes 50x50mm stencil data
• 20x20 holes 10x12mm stencil data
• First manufacturer at Taobao: with -+0.01mm error, CNY1200=$170 per piece

Deep learning droplet tracker

Install Python and Anaconda then move to jupyter directory

cd jupyter

jupyter notebook