Sampling space for specific genes about tybalt

[gwaybio]

Hi @spadavec , thanks for your interest in the project!

Yes, I imagine that this is possible. One could imagine taking a couple real samples, fixing all gene measurements except for one (or a few) and replacing them with some sampling distribution. Then, take the now "simulated" samples and compress and decompress them with the trained network.

The compression will inform how latent space features react to gene expression changes (and possibly identify a single or small group of modules that capture that specific signal) and, by comparing real output to decompressed output of simulated samples, the decompression will inform how specific genes are changing. This would depend on how well specific genes are reconstructed in the first place too!

We also haven't done this evaluation before, so this might not even work at all 🤷‍♂️. I think @cgreene and maybe @ajlee21 have thought about this question a bit more though!

[stale]

This issue has been automatically marked as stale because it has not had recent activity. It will be closed if no further activity occurs. Thank you for your contributions.

[spadavec]

After thinking about this some more, I believe the best approach is to just sample the decoder en masse, and find "samples" that have values for the particular gene within the desired range, and see how the other genes are expressed.

Closing.

[cgreene]

You might think about swapping out cross entropy. I think that's the main thing that keeps zero-one required.

On Wed, Mar 27, 2019, 11:41 AM Vito Spadavecchio ***@***.***> wrote: After thinking about this some more, I believe the best approach is to just sample the decoder *en masse*, and find "samples" that have values for the particular gene within the desired range, and see how the other genes are expressed. Closing. — You are receiving this because you were mentioned. Reply to this email directly, view it on GitHub <#145 (comment)>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AAhHs0KkXcuxoc-e6f5OXUSzV9TqIvY8ks5va59OgaJpZM4aJ5bu> .

-- Casey S. Greene, Ph.D. Associate Professor Dept. of Systems Pharmacology and Translational Therapeutics Perelman School of Medicine University of Pennsylvania web: http://www.greenelab.com phone: [phone] Director Childhood Cancer Data Lab Alex's Lemonade Stand Foundation web: http://ccdatalab.org