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Overlapper issue about apollo HOT 3 CLOSED

gmod avatar gmod commented on July 21, 2024
Overlapper issue

from apollo.

Comments (3)

nathandunn avatar nathandunn commented on July 21, 2024

I'm a bit confused, i thought you guys where using one of the overlappers : org.bbop.apollo.web.overlap.SimpleOverlapper org.bbop.apollo.web.overlap.OrfOverlapper but there's a number of overlapping models:

Scaffold72 cap_1 gene 61337 103237 . -1 . ID=0A07543993DACE8960CC9629545337DA;
Scaffold72 cap_1 mRNA 61337 103237 . -1 . ID=736A6FAB2711A89DF80DBD885018018D;Parent=0A07543993DACE8960CC9629545337DA;description=hypothetic
Scaffold72 cap_1 exon 102422 103237 . -1 . ID=ED463816EEEB100EB248C73421C16270;Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 exon 67474 67696 . -1 . ID=354E342636A42D8970CF82FC40C2D529;Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 exon 66692 66914 . -1 . ID=FE6B728A7E01F28597F183D0D6168146;Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 exon 61337 62041 . -1 . ID=47549428943A0B48665E3F23A7761B79;Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 CDS 102422 103237 . - 0 Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 CDS 67474 67696 . - 0 Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 CDS 66692 66914 . - 2 Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 CDS 61747 62041 . - 1 Parent=736A6FAB2711A89DF80DBD885018018D;
Scaffold72 cap_1 three_prime_utr 61337 61746 . - . Parent=736A6FAB2711A89DF80DBD885018018D

vs.

Scaffold72 cap_2 gene 61337 111778 . -1 . ID=24AEC9BEE634E7F79816137F5174AEA5;
Scaffold72 cap_2 mRNA 61337 111778 . -1 . ID=68E3F185F28BE0EEB731B75A2A658725;Parent=24AEC9BEE634E7F79816137F5174AEA5;description=hypothetic
Scaffold72 cap_2 exon 110969 111778 . -1 . ID=AC058E79BE0AEF8076A5D3FB84A447B3;Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 exon 67474 67696 . -1 . ID=1172DFD9559E544F2BBAAF2B559EB56D;Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 exon 66692 66914 . -1 . ID=7CAF1B65B5481734981C98A412F6A70D;Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 exon 61337 62041 . -1 . ID=B1865B0A8169B5207A4F86A187E0E8C5;Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 CDS 110969 111778 . - 0 Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 CDS 67474 67696 . - 0 Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 CDS 66692 66914 . - 2 Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 CDS 61747 62041 . - 1 Parent=68E3F185F28BE0EEB731B75A2A658725;
Scaffold72 cap_2 three_prime_utr 61337 61746 . - . Parent=68E3F185F28BE0EEB731B75A2A658725

two others also overlap these :

Scaffold72 cap_2 gene 61337 91196 . -1 . ID=24C0AA2B26260CE88282B6F421D1EB05;

Scaffold72 cap_2 gene 61337 123423 . -1 . ID=A5FAA5FB4BA934E7320D3A07EC99DE21;

and :

Scaffold253 cap_1 gene 712496 728328 . 1 . ID=E9392BD95E082DAFDFC472EE5134314E;
Scaffold253 cap_1 mRNA 721875 728328 . 1 . ID=4CE8F78E561F122CFA50599FE6E482B9;Parent=E9392BD95E082DAFDFC472EE5134314E;description=hypothetic
Scaffold253 cap_1 exon 721875 722744 . 1 . ID=973FDEBC3026A0BF3DA7ADF158B4973D;Parent=4CE8F78E561F122CFA50599FE6E482B9;
Scaffold253 cap_1 exon 727523 728328 . 1 . ID=ED3BAEC4827A6C5AC0C8FDF7745FEB67;Parent=4CE8F78E561F122CFA50599FE6E482B9;
Scaffold253 cap_1 CDS 721875 722744 . + 0 Parent=4CE8F78E561F122CFA50599FE6E482B9;
Scaffold253 cap_1 CDS 727523 727789 . + 0 Parent=4CE8F78E561F122CFA50599FE6E482B9;
Scaffold253 cap_1 three_prime_utr 727790 728328 . + . Parent=4CE8F78E561F122CFA50599FE6E482B9
Scaffold253 cap_1 mRNA 724599 728328 . 1 . ID=60820AA4F2F8A0AD46FBD047B5003DF5;Parent=E9392BD95E082DAFDFC472EE5134314E;description=hypothetic
Scaffold253 cap_1 exon 724599 725432 . 1 . ID=FECADB68CC0EB0AE77CDB39DB4EA2A4D;Parent=60820AA4F2F8A0AD46FBD047B5003DF5;
Scaffold253 cap_1 exon 727523 728328 . 1 . ID=ED3BAEC4827A6C5AC0C8FDF7745FEB67;Parent=60820AA4F2F8A0AD46FBD047B5003DF5;
Scaffold253 cap_1 CDS 724599 725432 . + 0 Parent=60820AA4F2F8A0AD46FBD047B5003DF5;
Scaffold253 cap_1 CDS 727523 727789 . + 0 Parent=60820AA4F2F8A0AD46FBD047B5003DF5;
Scaffold253 cap_1 three_prime_utr 727790 728328 . + . Parent=60820AA4F2F8A0AD46FBD047B5003DF5
Scaffold253 cap_1 mRNA 712496 728328 . 1 . ID=F62A7BCE25259DBCD5CFB720D7905F29;Parent=E9392BD95E082DAFDFC472EE5134314E;description=hypothetic
Scaffold253 cap_1 exon 712496 713353 . 1 . ID=9C0E959BF112890F6288D82AA77CAC0F;Parent=F62A7BCE25259DBCD5CFB720D7905F29;
Scaffold253 cap_1 exon 727523 728328 . 1 . ID=ED3BAEC4827A6C5AC0C8FDF7745FEB67;Parent=F62A7BCE25259DBCD5CFB720D7905F29;
Scaffold253 cap_1 CDS 712496 713353 . + 0 Parent=F62A7BCE25259DBCD5CFB720D7905F29;
Scaffold253 cap_1 CDS 727523 727789 . + 0 Parent=F62A7BCE25259DBCD5CFB720D7905F29;
Scaffold253 cap_1 three_prime_utr 727790 728328 . + . Parent=F62A7BCE25259DBCD5CFB720D7905F29
Scaffold253 cap_1 mRNA 715185 728328 . 1 . ID=AEFDA4030D213D85A56727C3D380C047;Parent=E9392BD95E082DAFDFC472EE5134314E;description=hypothetic
Scaffold253 cap_1 exon 715185 716003 . 1 . ID=99DBBAA73A7D0689A1149D079C85EF10;Parent=AEFDA4030D213D85A56727C3D380C047;
Scaffold253 cap_1 exon 727523 728328 . 1 . ID=ED3BAEC4827A6C5AC0C8FDF7745FEB67;Parent=AEFDA4030D213D85A56727C3D380C047;
Scaffold253 cap_1 CDS 715185 716003 . + 0 Parent=AEFDA4030D213D85A56727C3D380C047;
Scaffold253 cap_1 CDS 727523 727789 . + 0 Parent=AEFDA4030D213D85A56727C3D380C047;
Scaffold253 cap_1 three_prime_utr 727790 728328 . + . Parent=AEFDA4030D213D85A56727C3D380C047

#Comparison cap gene_2:

Scaffold253 cap_2 gene 720348 728328 . 1 . ID=B574E70F5CBFB92E086E33DB9A15D39F;
Scaffold253 cap_2 mRNA 720348 728328 . 1 . ID=8954245B7FE605AE3CE8C9FCE965727A;Parent=B574E70F5CBFB92E086E33DB9A15D39F;description=hypothetic
Scaffold253 cap_2 exon 720348 721199 . 1 . ID=52490E137AF3B6502F040C8198D0AF3A;Parent=8954245B7FE605AE3CE8C9FCE965727A;
Scaffold253 cap_2 exon 727523 728328 . 1 . ID=BCE699E12F75F2EEFEDC3088C2E98A7A;Parent=8954245B7FE605AE3CE8C9FCE965727A;
Scaffold253 cap_2 CDS 720348 721199 . + 0 Parent=8954245B7FE605AE3CE8C9FCE965727A;
Scaffold253 cap_2 CDS 727523 727789 . + 0 Parent=8954245B7FE605AE3CE8C9FCE965727A;
Scaffold253 cap_2 three_prime_utr 727790 728328 . + . Parent=8954245B7FE605AE3CE8C9FCE965727A

while this one i guess would indicate you're using the OrfOverlapper and not SimpleOveralapper

Scaffold47 cap_1 gene 555349 561633 . -1 . ID=A55D8834BEBA1336F794E99C4EE75959;
Scaffold47 cap_1 mRNA 555349 561633 . -1 . ID=6A9FD626E6489952BCC8BC671415A075;Parent=A55D8834BEBA1336F794E99C4EE75959;description=hypothetic
Scaffold47 cap_1 exon 561595 561633 . -1 . ID=6F48B53238FD3598B83F7B4BCBB4FD6B;Parent=6A9FD626E6489952BCC8BC671415A075;
Scaffold47 cap_1 exon 561288 561472 . -1 . ID=B11C765A0CE3C50C85185F4C75B297A6;Parent=6A9FD626E6489952BCC8BC671415A075;
Scaffold47 cap_1 exon 555349 555472 . -1 . ID=FA59A666FE1F7CB6FE52B310A339B608;Parent=6A9FD626E6489952BCC8BC671415A075;
Scaffold47 cap_1 five_prime_utr 561607 561633 . - . Parent=6A9FD626E6489952BCC8BC671415A075
Scaffold47 cap_1 CDS 561595 561606 . - 0 Parent=6A9FD626E6489952BCC8BC671415A075;
Scaffold47 cap_1 CDS 561288 561472 . - 0 Parent=6A9FD626E6489952BCC8BC671415A075;
Scaffold47 cap_1 CDS 555349 555472 . - 1 Parent=6A9FD626E6489952BCC8BC671415A075;

Scaffold47 cap_2 gene 560941 566415 . -1 . ID=5607EC6CA6BE52483F589600D74E7738;
Scaffold47 cap_2 mRNA 560941 566415 . -1 . ID=DCCC0F3F2BD93E8032ACDE3EEF48756E;Parent=5607EC6CA6BE52483F589600D74E7738;description=hypothetic
Scaffold47 cap_2 exon 566379 566415 . -1 . ID=D95314599F12FD86C925608EF3BD7349;Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 exon 566076 566260 . -1 . ID=56CF11742374F9C2BF86A636504E0C4C;Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 exon 565764 566011 . -1 . ID=5B6CA65BB179BE1DA06F34973DD7DFCB;Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 exon 563133 563158 . -1 . ID=FC68BE3C9BE667D9CCC7B010B51360FC;Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 exon 560941 561223 . -1 . ID=17A5D18F41B9473575043309AD07EBB0;Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 five_prime_utr 566394 566415 . - . Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E
Scaffold47 cap_2 CDS 566379 566393 . - 0 Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 CDS 566076 566260 . - 0 Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 CDS 565764 566011 . - 1 Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 CDS 563133 563158 . - 2 Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 CDS 561218 561223 . - 0 Parent=DCCC0F3F2BD93E8032ACDE3EEF48756E;
Scaffold47 cap_2 three_prime_utr 560941 561217 . - . Parent=DCCC0F3F2BD93E8032ACDE3EEF48756

from apollo.

nathandunn avatar nathandunn commented on July 21, 2024

Probably just need to double-check or clarify.

from apollo.

monicacecilia avatar monicacecilia commented on July 21, 2024

Unable to reproduce this.
Should it arise again after 2.0, let's bring it back. I'm closing for now.

from apollo.

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