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a Nextflow SNV calling and annotation pipeline based on DKFZ-ODCF/SNVCallingWorkflow

License: MIT License

HTML 0.57% R 20.09% Python 28.59% Perl 21.40% Shell 0.93% Groovy 7.48% Nextflow 20.79% Dockerfile 0.14%
snv snv-call snv-annotation dkfz ghga somatic-mutations somatic-variants odcf

nf-snvcalling's Issues

fix documents

Is your feature request related to a problem? Please describe

fix documents

Describe the solution you'd like

No response

Describe alternatives you've considered

No response

Additional context

No response

fix input checking

Is your feature request related to a problem? Please describe

no

Describe the solution you'd like

fix input_checking.nf check nf-aceseq pipeline

Describe alternatives you've considered

implement nf-validation plugin

Additional context

No response

Alternate Annovar with VEP

Is your feature request related to a problem? Please describe

There will be options for annotations to use with VEP

Describe the solution you'd like

add VEP module

Describe alternatives you've considered

No response

Additional context

No response

Rearrange source requirements

Is your feature request related to a problem? Please describe

Rearrange source requirements

Describe the solution you'd like

No response

Describe alternatives you've considered

No response

Additional context

No response

nf-core download do not download the singularity images

Have you checked the docs?

Description of the bug

I have used nfcore-download command as suggested to pull the singularity images. But resulted in errors (see terminal outputs)

Command used and terminal output

$nf-core download ghga-de/nf-snvcalling

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 2.14.1 - https://nf-co.re


WARNING  Could not find GitHub authentication token. Some API requests may fail.
? Select release / branch: v2.0.0  [release]
? Include the nf-core's default institutional configuration files into the download? Yes

In addition to the pipeline code, this tool can download software containers.
? Download software container images: singularity

If you are working on the same system where you will run Nextflow, you can amend the downloaded images to the ones in the$NXF_SINGULARITY_CACHEDIR folder, Nextflow will
automatically find them. However if you will transfer the downloaded files to a different system then they should be copied to the target folder.
? Copy singularity images from $NXF_SINGULARITY_CACHEDIR to the target folder or amend new images to the cache? amend

If transferring the downloaded files to another system, it can be convenient to have everything compressed in a single file.
This is not recommended when downloading Singularity images, as it can take a long time and saves very little space.
? Choose compression type: tar.gz
INFO     Saving 'ghga-de/nf-snvcalling'
          Pipeline revision: 'v2.0.0'
          Use containers: 'singularity'
          Container library: 'quay.io'
          Using $NXF_SINGULARITY_CACHEDIR': /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image'
          Output file: 'ghga-de-nf-snvcalling_v2.0.0.tar.gz'
          Include default institutional configuration: 'True'
INFO     Downloading centralised configs from GitHub
INFO     Downloading workflow files from GitHub
INFO     Processing workflow revision v2.0.0, found 8 container images in total.
Pulling singularity images ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━╸━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━  62% • 5/8 completed
kubran/bcftools:1.9 WARNING: SINGULARITY_CACHEDIR and APPTAINER_CACHEDIR have different values, using the latter ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ERROR    "kubran/bcftools:1.9" failed for unclear reasons.

INFO     Pulling of "kubran/bcftools:1.9" failed.
         Please troubleshoot the command
         "singularity pull --name /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-bcftools-1.9.img docker://quay.io/kubran/bcftools:1.9"
         manually.

ERROR    "kubran/bcftools:1.9" failed for unclear reasons.

ERROR    Pulling of "kubran/bcftools:1.9" failed.
         Please troubleshoot the command
         "singularity pull --name /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-bcftools-1.9.img docker://quay.io/kubran/bcftools:1.9"
         manually.

ERROR    Not able to pull image of ('kubran/bcftools:1.9', '/omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-bcftools-1.9.img', None).
Pulling singularity images ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━╸━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━  75% • 6/8 completed
kubran/odcf_mpileupsnvcalling:v0 WARNING: SINGULARITY_CACHEDIR and APPTAINER_CACHEDIR have different values, using the latter ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ERROR    "kubran/odcf_mpileupsnvcalling:v0" failed for unclear reasons.

INFO     Pulling of "kubran/odcf_mpileupsnvcalling:v0" failed.
         Please troubleshoot the command
         "singularity pull --name /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-odcf_mpileupsnvcalling-v0.img
         docker://quay.io/kubran/odcf_mpileupsnvcalling:v0" manually.

ERROR    "kubran/odcf_mpileupsnvcalling:v0" failed for unclear reasons.

ERROR    Pulling of "kubran/odcf_mpileupsnvcalling:v0" failed.
         Please troubleshoot the command
         "singularity pull --name /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-odcf_mpileupsnvcalling-v0.img
         docker://quay.io/kubran/odcf_mpileupsnvcalling:v0" manually.

ERROR    Not able to pull image of ('kubran/odcf_mpileupsnvcalling:v0',
         '/omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-odcf_mpileupsnvcalling-v0.img', None). Service might be down or internet
Pulling singularity images ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━╸━━━━━━━━━━━━━━━  88% • 7/8 completed
kubran/samtools:v1.9 WARNING: SINGULARITY_CACHEDIR and APPTAINER_CACHEDIR have different values, using the latter ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ERROR    "kubran/samtools:v1.9" failed for unclear reasons.

INFO     Pulling of "kubran/samtools:v1.9" failed.
         Please troubleshoot the command
         "singularity pull --name /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-samtools-v1.9.img
         docker://quay.io/kubran/samtools:v1.9" manually.

ERROR    "kubran/samtools:v1.9" failed for unclear reasons.

ERROR    Pulling of "kubran/samtools:v1.9" failed.
         Please troubleshoot the command
         "singularity pull --name /omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-samtools-v1.9.img
         docker://quay.io/kubran/samtools:v1.9" manually.

ERROR    Not able to pull image of ('kubran/samtools:v1.9', '/omics/groups/OE0526/internal/users/pritam/raredisease/singularity_image/kubran-samtools-v1.9.img', None).
Pulling singularity images ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 100% • 8/8 completed
INFO     Compressing output into archive
INFO     Command to extract files: tar -xzf ghga-de-nf-snvcalling_v2.0.0.tar.gz
INFO     MD5 checksum for 'ghga-de-nf-snvcalling_v2.0.0.tar.gz': f5abf45510cc5c85827e4e02597d0a65

Relevant files

No response

System information

Nextflow version 23.10.1
DKFZ Cluster
Singularity
CenOS

prettier for CI/CD

Is your feature request related to a problem? Please describe

install prettier for CI/CD checks

Describe the solution you'd like

No response

Describe alternatives you've considered

No response

Additional context

No response

generate test annotation files

Is your feature request related to a problem? Please describe

generate test annotation files for full test case run

Describe the solution you'd like

subtract regions in testdata.

Describe alternatives you've considered

No response

Additional context

No response

update nextflow_schema.json

Is your feature request related to a problem? Please describe

nextflow_schema.json missises some parameters

Describe the solution you'd like

No response

Describe alternatives you've considered

No response

Additional context

No response

Update README

Is your feature request related to a problem? Please describe

add

The reference set bundle which is used in PCAWG study can be found and downloaded here. (NOTE: only in hg19)

Describe the solution you'd like

No response

Describe alternatives you've considered

No response

Additional context

No response

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