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wes_srslyumi's Introduction

srslyumi instruction

useful website

things to do

  • source /isilon/RnD/tools/bcbio_072619/env.sh

  • source /isilon/prodx/bcl2fastq/2.20.0.422/env.sh

  • srslyumi /scratch/ngs_run_data/Sequencing_Raw_Data/NovaSeqOutput/220411_A01690_0016_AHTWVGDRXY /scratch/ngs_run_data/Sequencing_Raw_Data/NovaSeqOutput/220411_A01690_0016_AHTWVGDRXY/Data/Intensities/BaseCalls/FASTQ

  • python library location: /isilon/RnD/bcbio-tool-data/anaconda/lib/python3.6/site-packages/srslyumi/

    • updating with --no-lane-splitting in /isilon/RnD/bcbio-tool-data/anaconda/lib/python3.6/site-packages/srslyumi/cli.py

  • fix the UMI in the read name to be compatible with bcbio

    • in the FASTQ folder, do /isilon/RnD/tools/custom_script/wes/fastq_umi_fix.sh

wes_srslyumi's People

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