Comments (2)
RefMasker is a module that masks repetitive regions in the reference genome, rather than recombinant regions. The RecHMM and RecFilter modules are designed to do similar things as "Gubbins" and "ClonalFrame". They are somewhat unrelated to what you want.
In EnteroBase, we separate RefMasker, RefMapper and RefMapper_matrix as individual procedures, because we want to save intemediate files, which can be reused later.
Here as a standalone version package 'EToKi', the three steps in EnteroBase are concatenated together into "EToKi align". You do not need to run them separately (although you can still do that).
For your use, you can just run EToKi align, and put reference and queried genomes in, "EToKi align" will (1) mask the reference, (2) align queried genomes into reference, and (3) generate the SNP matrix.
The output of EToKi align can then be fed into "EToKi phylo" to get the ML tree.
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Thanks so much!
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Related Issues (11)
- Make pip installable HOT 1
- Usearch is proprietary HOT 2
- --install --path HOT 1
- Need documentation on cgMLST HOT 1
- Phylo.py core genome output
- Use a single tool with subcommands
- requests HOT 1
- Doesn't handle Help when no module is given HOT 1
- Error when running example script EToKi.py MLSTdb
- Error when downloading spades dependency
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