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Digital Sorting Algorithm
Dear the owner: When applying the DSA algorithm to my microarray data of RNA expression from nasopharygnx (NP) specimens that contains two major cell types: epithelial and blood/immune cells, I got the estimated weight/proportion for epithelial cells greater than 1 in several of the 26 NP samples (see attached file). Is this normal? Should I re-scale the 2 weights (epithelial and blood) for a sample to a sum of 1 (100%)? I tried the re-scale, and the overall estimates across 4 groups of samples looked very close to the reality biologically, i.e. that more infection, the less epithelial cells in the epithelial-blood mixed samples. I am here with the hope to get an expert opinion on how to deal with this issue with the DSA package. Thank you!
estimated_weight_QLM_157markers.xlsx
Using both R studio & R x64 3.1.1
Package not recognised in appropriate directory on computer where I placed it (i.e. not available to be selected). Have done this for other packages in the past with no problems. Am I missing something here?
Many thanks for your help
It looks like .tar.gz file is broken.
Anyway it would be better to put here unpacked source files.
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