Comments (5)
Dear @NicMAlexandre,
There really isn’t anything formal that I ever wrote. Your specific application matters quite a bit. Not knowing anything else, I would recommend FDR for BUSTED. aBSREL is a bit more complicated, because it does multiple testing correction at the level of branches for each run. You can layer another correction on top of that if you want, probably another FDR. Can you give me any details?
Best,
Sergei
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Dear @NicMAlexandre,
aBSREL
already does the within-gene correction for you, so that's not something you need to do after the fact. The number of test branches for BUSTED
is immaterial for the purposes of per-gene correction (one gene = one test), only inasmuch as you would expect very low power to reject given a single foreground branch. In other words, you would expect the actual Type I error rate to be much lower than the nominal one (p-value cutoff). Doing a multiple test correction here is probably going to dilute everything out.
Would you happen to have a list of p-values (for the 11k genes) that BUSTED produced that you could share?
Best,
Sergei
from hyphy.
Thank you, that makes complete sense. I just direct messaged the P-values.
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Stale issue message
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Related Issues (20)
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