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This is the organization and research for the UF/IFAS Industrial Hemp Pilot Project

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hemp's Introduction

This is the organization and research for the UF/IFAS Industrial Hemp Pilot Project

Hemp Metadata and Field Notes

Dates and season of each hemp trial conducted at TREC, Homestead, FL.

PilotPlot1; May 1st, 2019 (Summer)
PilotPlot2; June 21st, 2019 (Summer)
VarietyTrial1; May 22nd, 2019 (Summer)
PilotPlotPlus8; July 18th, 2019 (Summer)

PilotPlot1_summer_2019_Emergence.csv: Emergence data of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Row number in the measurement zone of each experimental unit (1: left; 2: right).
Emergence: Number of plants that have emerged from the soil as a result of planting (Planting date: May 1st, 2019).
Notes: - Blanks due to missing data
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
- Block 1: Row 1 - Column D, Row 2 - Column A
- Block 2: Row 3 - Column D, Row 4 - Column D
- Block 3: Row 2 - Column A, and C
- Block 4: Row 3 - Column A, Row 4 - Column C

PilotPlot1_summer_2019_StandCount.csv: Population quantification of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
StandCount: Total amount of plants in experimental unit.
Notes: - Blanks due to missing data
- Block 3 - Row 2 - Column A: Plants did not emerge.
- Block 3 - Row 2 - Column C: Experimental unit was mixed with adjacent variety.

PilotPlot1_summer_2019_Flowering.csv: Flowering data file of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Induc_perc: Percentage of flowering induction
MaleOpen_perc: Percent of male plants with over half of flowers opened.
FemaleOpen_perc: Percent of female plants with over half of flowers opened
MonoOpen_perc: Percent of monoecious plants with over half of female flowers opened.
SeedHard_perc: Percent of seed hardening.
Notes: - Blanks due to missing data
- Block 3 - Row 2 - Column A: Plants did not emerge.
- Block 3 - Row 2 - Column C: Experimental unit was mixed with adjacent variety.

PilotPlot1_summer_2019_PlantDensity.csv: Plant density data file of the first pilot plot

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Row number in the measurement zone of each experimental unit (1: left; 2: right).
Quantity: Amount of plants standing within the measurement zone.
Notes: - Blanks due to missing data
- Block 3 - Row 2 - Column A: Plants did not emerge.
- Block 3 - Row 2 - Column C: Experimental unit was mixed with adjacent variety.
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 1: Row 1 - Column D, Row 2 - Column A
  - Block 2: Row 3 - Column D, Row 4 - Column D
  - Block 4: Row 3 - Column A, Row 4 - Column C -From July 31st on, the following blocks were not measured for plant density because they reached 100% seed hardness.
  - Block 1: Row 2 - Column C
  - Block 2: Row 4 - Column A
  - Block 3: Row 1 - Column D; Row 2 - Column A, C, and D
  - Block 4: Row 3 - Column B

PilotPlot1_summer_2019_PlantHeight-SexRatio.csv: Plant height and sex ratio file of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the measurement zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Sex: Sexual identification of the plant.
- V: Vegetative stage
- M: Male
- F: Female
- C: Monoecious (0: undetermined; 1: 80 % male; 2: 60% male; 3: 50% male; 4: 60% female; 5: 80% female)
Notes: - Blanks due to missing data
- Block 3 - Row 2 - Column A: Plants did not emerge.
- Block 3 - Row 2 - Column C: Experimental unit was mixed with adjacent variety.
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 1: Row 1 - Column D, Row 2 - Column A
  - Block 2: Row 3 - Column D, Row 4 - Column D
  - Block 4: Row 3 - Column A, Row 4 - Column C
- From July 24th on, the following blocks were not measured for height and ratio because they reached 100% seed hardness.
  - Block 1: Row 2 - Column B and C.
  - Block 2: Row 4 - Column A
  - Block 3: Row 1 - Column D, Row 2 - Column D
  - Block 4: Row 3 - Column B, Row 4 - Column A
- From July 31st on, the additional following varieties were not measured for height and sex ratio because they reached 100% seed hardness.
  - Block 1: Row 2 - Column D
  - Block 2: Row 3 - Column B and C
  - Block 3: Row 1 - Column A
  - Block 4: Row 4 - Column B

PilotPlot1_summer_2019_GrainHarvest.csv: Grain harvest file of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
NumberOfPlants: Individuals within the harvest zone (1 m2) of each experimental unit.
BiomassWeight_kg: Weight of plants' biomass.
GrainFreshWeight_kg: Weight of threshed seeds.
GrainDryWeight_g: Weight of dry seeds, previously threshed.
CleanedHarvest_g: Weight of seeds after threshing, drying, and chaff removal.
RootWeight_g: Weight of plants' roots in grams.
Notes: - Blanks due to missing dataBlanks due to missing data
- Cleaned harvest was ONLY recorded for the following:
  - Block 3 - Row 1 - Column D
  - Block 2 - Row 3 - Column C
- Block 2 - Row 3 - Column C: Grain fresh weight, and root weight were not collected.

PilotPlot1_summer_2019_FiberHarvest_GM.csv: Fiber harvest (Group measurements) file of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
NumberOfPlants: Individuals within the harvest zone (1 m2) of each experimental unit.
FreshWeight_kg: Weight of plants' biomass.
MassOfLeaves_g: Weight of plants' leaves and branches.
SampleFreshWeight_g: Weight of 5 sampled stems.
SampleDryWeight_g: Weight of 5 dry sampled stems.
Notes: -Blanks due to missing data
- Sample dry weight to be recorded for the following:
  - Block 1 - Row 1 - Column C
  - Block 2 - Row 4 - Column B
  - Block 3 - Row 2 - Column B
  - Block 4 - Row 3 - Column D

PilotPlot1_summer_2019_FiberHarvest_IM.csv: Fiber harvest (Individual measurements) file of the first pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the harvest zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Diameter_cm: Stem diameter in centimeter at 30 cm from plant base.
Sex: Sexual identification of the plant.
- V: Vegetative stage
- M: Male
- F: Female
Notes: - Blanks due to missing data
- Block 1 - Row 1 - Column A - Rep 3: Missed data.
- Sex data was not recorded for the following:
  - Block 2 - Row 3 - Column A
  - Block 2 - Row 4 - Column B

VarietyTrial1_summer_2019_Emergence.csv: Emergence data of the first variety trial.

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Row number in the measurement zone of each experimental unit (1: left; 2: right).
Emergence: Number of plants that have emerged from the soil as a result of planting within the measurement zone (Planting date: May 22nd, 2019).
Notes: - Blanks due to missing data
- Block 6, 7, and 8 - Row 5 - Column D: Left blank on purpose.
- Block 7 - Row 2 - Column A: Mixed planting/missed data.
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 5: Row 2 - Column C, and D.
  - Block 6: Row 1 - Column B, Row 2 - Column D
  - Block 7: Row 3 - Column C, Row 4 - Column A
  - Block 8: Row 3 - Column C, Row 4 - Column C

VarietyTrial1_summer_2019_StandCount.csv: Population quantification of the first variety trial.

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
StandCount: Total amount of plants in experimental unit.
Notes: - Blanks due to missing data
- Block 6, 7, and 8 - Row 5 - Column D: Left blank on purpose.
- Block 7 - Row 2 - Column A: Mixed planting/missed data.

VarietyTrial1_summer_2019_Plant Density.csv: Plant density data file of the first variety trial. Data was only taken for the varieties studied in the pilot plot.

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Row number in the measurement zone of each experimental unit (1: left; 2: right).
Quantity: Amount of plants standing within the measurement zone.
Notes: - Blanks due to missing data.
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 5: Row 2 - Column C, and D.
  - Block 6: Row 1 - Column B, Row 2 - Column D
  - Block 7: Row 3 - Column C, Row 4 - Column A
  - Block 8: Row 3 - Column C, Row 4 - Column C
- From July 31st on, the following blocks were not measured for plant density because they reached 100% seed hardness.
  - Block 5: Row 4 - Column D
  - Block 6: Row 1 - Column C
  - Block 7: Row 5 - Column C
  - Block 8: Row 3 - Column D

VarietyTrial1_summer_2019_Flowering.csv: Flowering data of the first variety trial

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Induc_perc: Percentage of flowering induction
MaleOpen_perc: Percent of male plants with over half of flowers opened.
FemaleOpen_perc: Percent of female plants with over half of flowers opened
MonoOpen_perc: Percent of monoecious plants with over half of female flowers opened.
SeedHard_perc: Percent of seed hardening.
Notes: - Blanks due to missing data
- Block 6, 7, and 8 - Row 5 - Column D: Left blank on purpose
- From Jun 19th on, Block 7 - Row 2 - Column A: Experimental unit looked as mixed planting/missed data
- On June 26th, Block 7 - Row 3 - Column A : Missed data
- On Aug 12th, Block 6 - Row 4 - Column B and C: Missed data on SeedHard_perc.

VarietyTrial1_summer_2019_ PlantHeight.csv: Plant Height data of the first variety trial. 4 data points were taken in the whole each experimental unit regardless of the measurement zone as emergence was scattered.

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the measurement zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Notes: - Blanks due to missing data -Block 6, 7, and 8 - Row 5 - Column D: Left blank on purpose.
- On June 19th, Block 7 - Row 2 - Column A: Data might be representative of a mixed planting.
- From June 26th on, Block 7 - Row 3 - Column A: Mixed planting/missed data.
- From July 24th on, the following blocks were not measured for plant height because they reached 100% seed hardness.
  - Block 5:
    - Row 1 - Column B and C
    - Row 2 - Column B
    - Row 4 - Column B and D
    - Row 5 - Column B
  - Block 6
    - Row 1 - Column A and C
    - Row 2 - Column B
    - Row 3 - Column B and D (Unit D was 90% seed hardness on July 26th, 29th, 31th)
    - Row 5 - Column A and B
  - Block 7
    - Row 2 - Column D
    - Row 3 - Column A
    - Row 4 - Column B and C
    - Row 5 - Column B and C
  - Block 8
    - Row 2 - Column D
    - Row 3 - Column A and D
    - Row 4 - Column D
    - Row 5 - Column A and B
- From August 9th on, the following blocks were not measured for plant height because they reached 100% seed hardness.
  - Block 7
    - Row 1 - Column B
  - Block 8
    - Row 4 - Column B
- From August 20th on, the following blocks were not measured for plant height because they reached 100% seed hardness.
  - Block 5
    - Row 1 - Column A
    - Row 3 - Column A and B
  - Block 6
    - Row 3 - Column A
    - Row 4 - Column C
  - Block 7
    - Row 2 - Column B and C
  - Block 8
    - Row 2 - Column C
    - Row 5 - Column C

VarietyTrial1_summer_2019_ SexRatio.csv: Sex data of the first variety trial.

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Male_perc: Percent of male plants.
Female_perc: Percent of female plants.
Mono_perc: Percent of monoecious plants.
Veg_perc: Percent of plants in vegetative stage.
Notes: - Blanks due to missing data
- Block 6, 7, and 8 - Row 5 - Column D: Left blank on purpose.
- Block 7 - Row 2 - Column A: Mixed planting/Missed data
- From July 24th on, the following blocks were not measured for plant height because they reached 100% seed hardness.
  - Block 5:
    - Row 1 - Column B and C
    - Row 2 - Column B
    - Row 4 - Column B and D
    - Row 5 - Column B
  - Block 6
    - Row 1 - Column A and C
    - Row 2 - Column B
    - Row 3 - Column B and D
    - Row 5 - Column A and B
  - Block 7
    - Row 2 - Column D
    - Row 3 - Column A
    - Row 4 - Column B and C
    - Row 5 - Column B and C
  - Block 8
    - Row 2 - Column D
    - Row 3 - Column A and D
    - Row 4 - Column D
    - Row 5 - Column A and B
- From August 9th on, the following blocks were not measured for sex ratio because they reached 100% seed hardness.
  - Block 7
    - Row 1 - Column B
  - Block 8
    - Row 4 - Column B
- From August 20th on, the following blocks were not measured for sex ratio because they reached 100% seed hardness.
  - Block 5
    - Row 1 - Column A
    - Row 3 - Column A and B
  - Block 6
    - Row 3 - Column A
    - Row 4 - Column C
  - Block 7
    - Row 2 - Column B and C
  - Block 8
    - Row 2 - Column C
    - Row 5 - Column C

VarietyTrial1_summer_2019_PlanHeight-SexRatioPP.csv: Plant height and sex ratio file of the first variety trial. Data was only taken for the varieties studied in the pilot plot.

Block: Block composed of 19 varieties, which was replicated 4 times
- Block 9 is composed of 4 additional fiber varieties replicated 4 times in the same block.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the measurement zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Sex: Sexual identification of the plant.
- V: Vegetative stage
- M: Male
- F: Female
- C: Monoecious (0: undetermined; 1: 80 % male; 2: 60% male; 3: 50% male; 4: 60% female; 5: 80% female)
Notes: - Blanks due to missing data
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 5: Row 2 - Column C, and D.
  - Block 6: Row 1 - Column B, Row 2 - Column D
  - Block 7: Row 3 - Column C, Row 4 - Column A
  - Block 8: Row 3 - Column C, Row 4 - Column C
- From July 31st on, the following blocks were not measured for plant height because they reached 100% seed hardness.
  - Block 5: Row 4 - Column D
  - Block 6: Row 1 - Column C
  - Block 7: Row 5 - Column C
  - Block 8: Row 3 - Column D
- On August 8th, data was recorded on Block 5 - Row 4 - Column D. Need to be deleted?

VarietyTrial1_summer_2019_GrainHarvest.csv: Grain harvest file of the first variety trial.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
NumberOfPlants: Individuals within the harvest zone (1 m2) of each experimental unit.
BiomassWeight_kg: Weight of plants' biomass.
GrainFreshWeight_kg: Weight of threshed seeds.
GrainDryWeight_g: Weight of dry seeds, previously threshed.
CleanedHarvest_g: Weight of seeds after threshing, drying, and chaff removal
RootWeight_g: Weight of plants' roots in grams
Notes: - Blanks due to missing data
- Grain dry weight and cleaned harvest data to be recorded
  - Block 5
    - Row 3 - Column C
    - Row 1 - Column A
    - Row 3 - Column B
    - Row 3 - Column D
  - Block 6
    - Row 4 - Column B
    - Row 3 - Column D
    - Row 4 - Column C
  - Block 7
    - Row 1 - Column D
    - Row 1 - Column B
    - Row 2 - Column B
  - Block 8
    - Row 4 - Column A
    - Row 4 - Column B
    - Row 2 - Column C
    - Row 1 - Column B
- Block 7 - Row 2 - Column A was not measured due to mixed planting in the field

VarietyTrial1_summer_2019_FiberHarvest_GM.csv: Fiber harvest (Group measurements) file of the first variety trial.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
NumberOfPlants: Individuals within the harvest zone (1 m2) of each experimental unit.
FreshWeight_kg: Weight of plants' biomass.
MassOfLeaves_g: Weight of plants' leaves and branches.
SampleFreshWeight_g: Weight of 5 sampled stems.
SampleDryWeight_g: Weight of 5 dry sampled stems.
Notes: - Blanks due to missing data
- Sample dry weight to be recorded for the following:
  - Block 5
    - Row 1 - Column D
    - Row 5 - Column C
    - Row 5 - Column A
  - Block 6
    - Row 2 - Column C
    - Row 3 - Column C
    - Row 4 - Column D
  - Block 7
    - Row 5 - Column A
    - Row 3 - Column D
    - Row 1 - Column A
  - Block 8
    - Row 2 - Column A
    - Row 1 - Column C
    - Row 2 - Column B
  - Block 9
    - Row 1 - Column B
    - Row 2 - Column B
    - Row 3 - Column D
    - Row 4 - Column C

VarietyTrial1_summer_2019_FiberHarvest_IM.csv: Fiber harvest (Individual measurements) file of the first variety trial.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the harvest zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Diameter_cm: Stem diameter in centimeter at 30 cm from plant base.
Sex: Sexual identification of the plant.
- V: Vegetative stage
- M: Male
- F: Female -Notes: - Blanks due to missing data
- No missing data

PilotPlot2_summer_2019_Emergence.csv: Emergence data of the second pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Row number in the measurement zone of each experimental unit (1: left; 2: right).
Emergence: Number of plants that have emerged from the soil as a result of planting (Planting date: June 21st, 2019).
Notes: - Blanks due to missing data
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 1: Row 1 - Column D, Row 2 - Column A
  - Block 2: Row 3 - Column D, Row 4 - Column D
  - Block 3: Row 2 - Column A, and C
  - Block 4: Row 3 - Column A, Row 4 - Column C

PilotPlot2_summer_2019_StandCount.csv: Population quantification of the second pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
StandCount: Total amount of plants in experimental unit.
Notes: - Blanks due to missing data
- No missing data

PilotPlot2_summer_2019_Flowering.csv: Flowering data file of the second pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Induc_perc: Percentage of flowering induction
MaleOpen_perc: Percent of male plants with over half of flowers opened.
FemaleOpen_perc: Percent of female plants with over half of flowers opened
MonoOpen_perc: Percent of monoecious plants with over half of female flowers opened.
SeedHard_perc: Percent of seed hardening. -Notes: -Blanks due to missing data
- From Jun 19th on, the following blocks were not measured due to
  - Block 1 - Row 1 - Column D
  - Block 3 - Row 2 - Column C -On July 29th, Block 3 - Row 1 - Column A: Missed data

PilotPlot2_summer_2019_PlantHeight-SexRatio.csv: Plant height and sex ratio file of the second pilot plot.

Block: Block composed of 8 varieties, which was replicated 4 times
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the measurement zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Sex: Sexual identification of the plant.
- V: Vegetative stage
- M: Male
- F: Female
- C: Monoecious (0: undetermined; 1: 80 % male; 2: 60% male; 3: 50% male; 4: 60% female; 5: 80% female)
Notes: - Blanks due to missing data
- Block 1 - Row 1 - Column D: Plants did not emerge.
- Block 3 - Row 2 - Column C: Plants did not emerge.
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 1: Row 1 - Column D, Row 2 - Column A
  - Block 2: Row 3 - Column D, Row 4 - Column D
  - Block 3: Row 2 - Column A, and C
  - Block 4: Row 3 - Column A, Row 4 - Column C

PilotPlotPlus8_summer_2019_Emergence.csv: Emergence data of the fourth planting.

Block: Block composed of 8 varieties, which was replicated 4 times
- Block 10-13: Set of pilot plot varieties; Block 14-17: Set of varieties from variety trial
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Row number in the measurement zone of each experimental unit (1: left; 2: right).
Emergence: Number of plants that have emerged from the soil as a result of planting (Planting date: July 18th, 2019).
Notes: - Blanks due to missing data
- Due to the CBD planting design, numbers in the following experimental units represent the total amounts of CBD plants in it. No measurement zone was established for them unlike the other varieties.
  - Block 10: Row 1 - Column D, Row 2 - Column A
  - Block 11: Row 3 - Column D, Row 4 - Column D
  - Block 12: Row 2 - Column A, and C.
  - Block 13: Row 3 - Column A, Row 4 - Column C
  - Block 14: Row 2 - Column B, C, and D
  - Block 15: Row 3 - Column B and D, Row 4 - Column A
  - Block 16: Row 1 - Column C, Row 2 - Column C and D
  - Block 17: Row 3 - Column B and C, Row 4 - Column D

PilotPlotPlus8_summer_2019_StandCount.csv: Population quantification of the fourth planting.

Block: Block composed of 8 varieties, which was replicated 4 times
- Block 10-13: Set of pilot plot varieties; Block 14-17: Set of varieties from variety trial
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
StandCount: Total amount of plants in experimental unit.
Notes: Blanks due to missing data
- No day was established on the recorded sheet - Maybe July 7th?
- No missing data

PilotPlotPlus8_summer_2019_Flowering.csv: Flowering data file of the fourth planting.

Block: Block composed of 8 varieties, which was replicated 4 times
- Block 10-13: Set of pilot plot varieties; Block 14-17: Set of varieties from variety trial
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Induc_perc: Percentage of flowering induction
MaleOpen_perc: Percent of male plants with over half of flowers opened.
FemaleOpen_perc: Percent of female plants with over half of flowers opened
MonoOpen_perc: Percent of monoecious plants with over half of female flowers opened.
SeedHard_perc: Percent of seed hardening. -Notes: -Blanks due to missing data
- From Aug 7thon, Block 15 - Row 3 - Column B and D: plants did not emerge
- On Aug 9th, Block 14 to 17 data was not recorded: missed data
- From Aug 12th on, the following observational units were not measured because of no plants
  - Block 12 - Row 1 - Column D
  - Block 15 - Row 3 - Column A
  - Block 16 - Row 2 - Column D
  - Block 17 - Row 3 - Column C
- From Aug 21st on, Block 14 - Row 1 - Column B was not measured because of no plants
- From Aug 23rd on, Block 16 - Row 2 - Column D: 1 plant appeared
- From Aug 26th on, the following blocks were not measured because of no plants
  - Block 10 - Row 1 - Column D
  - Block 16 - Row 2 - Columns C
- On Sept 9th, Block 14 - Row 2 - Column C: Missed data
- On Sept 11th, Block 13 - Row 4 - Column C: Missed data

PilotPlotPlus8_summer_2019_PlantHeight-SexRatio.csv: Plant height and sex ratio file of the fourth planting.

Block: Block composed of 8 varieties, which was replicated 4 times
- ONLY Block 10-13: Set of pilot plot varieties
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the measurement zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Sex: Sexual identification of the plant.
- V: Vegetative stage
- M: Male
- F: Female
- C: Monoecious (0: undetermined; 1: 80 % male; 2: 60% male; 3: 50% male; 4: 60% female; 5: 80% female)
Notes: - Blanks due to missing data
- Block 12 - Row 1 - Column D: Plants did not emerge
- On Oct 15th, Block 10 - Row 2 - Column C was not measured because of no plants.

PilotPlotPlus8_summer_2019_PlantHeight.csv: Plant height file of the fourth planting.

Block: Block composed of 8 varieties, which was replicated 4 times
- ONLY Block 14-17: Set of varieties from variety trial
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Rep: Individuals within the measurement zone of each experimental unit.
Height_cm: Stem length in centimeters from plant base to apex.
Notes: - Blanks due to missing data
- The following observational units were not measured because of no plants.
  - Block 14
    - Row 1 - Column A and B
    - Row 2 - Column B and D
  - Block 15
    - Row 3 - Column A, B, and D
  - Block 16
    - Row 1 - Column A
    - Row 2 - Column C and D
  - Block 17
    - Row 3 - Column C
    - Row 4 - Column B

PilotPlotPlus8_summer_2019_SexRatio.csv: Sex ratio file of the fourth planting.

Block: Block composed of 8 varieties, which was replicated 4 times
- ONLY Block 14-17: Set of varieties from variety trial
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Male_perc: Percent of male plants.
Female_perc: Percent of female plants.
Mono_perc: Percent of monoecious plants.
Veg_perc: Percent of plants in vegetative stage.
Notes: - Blanks due to missing data
- The following observational units were not measured because of no plants.
  - Block 14
    - Row 1 - Column A and B
    - Row 2 - Column B and D
  - Block 15
    - Row 3 - Column A, B, and D
  - Block 16
    - Row 1 - Column A
    - Row 2 - Column C and D
  - Block 17
    - Row 3 - Column C
    - Row 4 - Column B

hemp's People

Contributors

Stargazers

Watchers

Forkers

lmonserrate wdlngtn brymz danielcalza frameculture hemprinasocialproducts ajitwx

hemp's Issues

Project Initiation Checklist - Essential oil N Study

Upload Season 1 Data

Variety by planting location table
Complete planting date table

Add to list as per #16. Comment here as an update on progress.

Essential oil N Study - Project Preparation 2021 SOP

Project Objectives - To determine EO hemp (Cannabis sativa) response to applied nitrogen.

Project Description - Essential oil N Study
Project Protocol - 3 varieties x 6 rates x 4 reps. (18 trt).

Field size: L 242' x W 160’. (0.89 ac).

Plot size: L 25' x W 12'. (300 sqft).

Field Design - RCB with 4 reps. Blocked by replication.
Field Diagrams - Maps can be found on TEAMS.
Projected Start Date- Mid-April, Early-May.
Projected End Date- September/October.
Project Manager(s)- Daniel, Ajit.
Projected Planting Date- May 6st, 2021 (5/6/2021).
Crop(s)- Hemp: Essential oil - cv. Wife (CBD), CBG ICE (CBG), Maverick (Dual)
Irrigation - Drip-tape and possibly Solid set: over-head sprinklers.
Applications - Six (6) Nitrogen treatments, split between **four (4) applications.
Planting methods - (Wife and CBG ICE) - 3' Mounds, planted in center 1.25'. Transplanted by hand.
- (Maverick)- 3' Mounds, planted offset from center. Seeded by hand.
Data Collection & Sampling -
Project Maintenance -
Equipment needed - Moldboard plow, Harrow disk, raised bed shaper, roto-tiller, herbicide wagon, others - TBD.
Field crew assistance - Yes. For irrigation and land preparation.

Land Request

Preferred block- Block 10.
Section- Eastside.
Area/Acres requested- L 242' x W 160’. (0.89 ac).
Greenhouse - Yes.

To Do: Update check list

-[] Complete TREC Land Request

-[] Complete TREC Greenhouse Request

-[] Request faculty sponsor to complete UFarm request.

Land Preparation

Cultivation (mowing, moldboard plow, disc and level the field, form raised beds, drilling holes, etc.) -
Row Length - 25 ft.
In-Row Spacing - 8.5 inches for direct seed. 6 plants for transplants.
Between-Row Spacing - 6 ft.
Field Buffers - 30 ft.
Seeding? (Direct, Broadcast, Incorporated, Drill, Earthway, etc.) - direct.
Transplants? - Yes.
Support Structure (Rebar, String, etc.) - Yes, bamboo for transplants.
Plastic Mulch? - NA, paper mulch will be used on transplants.
Full image of field layout, design, and plot arrangement - Maps and layouts can be found on TEAMS.
Date to complete land preparation - 5/1/2021.

To Do: Update check list

-[] Complete TREC field preparation request

-[] Row Crop

-[] Plastic Culture

-[] Fruit Crop

Irrigation

Drip Tape? (drip placement, emitter spacing, flow-rate GPM/100 ft., etc.) - Drip tape. 2 lines per bed*?
Solid set? (overhead irrigation, microsprinkler) - Solid set - overhead.
Irrigation Timing (frequency and trigger) - As needed** [Once per week for first 2-3 weeks to germinate and establish**].
Irrigation Amount (per application) - 1 inch.

To Do: Update check list

-[] Complete TREC fieldwork request for irrigation.

Fertilizer Application

Label Name - Urea (N), Triple Super Phosphate (P), Sulfate of Potash (K)
Analysis rates (NPK, etc.) - 46-0-0 (N), 0-46-0 (P), 0-0-50 (K)
Application rate per acre -
Urea

```
 0 lbs N/ac 
```
50 lbs N/ac
100 lbs N/ac
150 lbs N/ac
200 lbs N/ac
250 lbs N/ac.
TSP
```
 60 lbs P2O5/ac
```
SOP
```
 300 lbs K2O/ac
```

Application method (banded on top of the bed, soil incorporation, etc.) - Pre-plant incorporated - except nitrogen.
Application timing and frequency - TBD.
Lab or field personnel - Ajit, Daniel.

To Do: Update check list

-[] Fertilizer request form

Herbicide Application

Label Name - Dual Magnum.
Active ingredient(s) and concentration - S-metolachlor, benoxacor.
Resistance Management: Group 15 Herbicide.
Restricted Entry Interval (REI): 24 hours.
Application rate per acre - 1 pint.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - pre-planting, sprayer.
Application timing, frequency, and location - Once at pre-planting. Only in the buffer rows.
Lab or field personnel - Daniel, Ajit, Jacob.

Pesticide Application

Label Name - NA.
Active ingredient(s) and concentration - NA.
Application rate per acre - NA.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - NA.
Application timing and frequency - NA.
Lab or field personnel - NA.

To Do: Update check list

-[] Worker Protection Standard Training

-[] Review "Training Workers and Handlers under the 2016 Revision Requirements" by Frederick M. Fishel.

-[] Found in the TREC network Unit drive > TREC Pesticide Use information > WPS information

-[] Pesticide request form

Project Maintenance Events

Research treatment application - Six (6) Nitrogen treatments, split between **four (4) applications. - TBD.
Weeding - PRE: Dual Magnum.** POST: cultivation or hooded applications.
Scouting - Weekly.
Sampling -
Harvesting and Sampling -
Project removal -

To Do: Update check list

-[] Equipment Request

-[] Complete TREC fieldwork request well ahead of maintenance events.

Data Collection & Sampling

Database - TEAMS, GitHub.
Data type -

Rainfall, irrigation, temperature
Soil moisture
Mehlich 3 P, pre-plant, 0-6”, block-level
Total N (dry combustion), pre-plant, 0-6”, block-level
NO3- & NH4+, pre-plant, 0-6”, block-level
SOM, pre-plant, 0-6”, block-level
Soil water holding capacity
Plants/m2 after emergence
Leaf tissue nutrient concentrations (N & P) i. Stage 1 ii. Stage 2 iii. Stage 3 iv. Stage 4 v. Stage 5
```
Biomass at harvest 
```
```
Yield , moisture, test weight 
```

Mehlich 3 P, post-harvest, 0-6”, plot-level

Total N (dry combustion), post-harvest, 0-6”, plot-level

NO3- & NH4+, post-harvest, 0-6”, plot-level

```
SOM, post-harvest, 0-6”, plot-level.
```

Data storage -
Metadata -
Data collection -
Protocols -
Timing -
Logistics -
Sample processing and analysis -

Contingencies

What important decisions are next to be made or remain a challenge?
What could possibly go wrong?

Clean messy data

Month to integer
Block as 1:21
VarietyTrial1_summer_2019_Emergence.csv
- Column mispelled
- 2 entries for Day
Flowering extra column, PilotPlot2$Block

Clean up flowering time data

Grain & Fiber N study - Project Preparation 2021 SOP

Project Objectives - To determine hemp (Cannabis sativa) response to applied nitrogen.

Project Description - Grain & Fiber N study
Project Protocol - 3 varieties x 6 rates x 4 reps. (18 trt).
Field Design - RCB with 4 reps. Blocked by replication.

Field size: L 242' x W 160’. (0.89 ac).

Plot size: L 25' x W 12'. (300 sqft).

Field Diagrams - Maps can be found on TEAMS.
Treatments - eighteen (18) experimental units.
Projected Start Date- Mid-April, Early-May.
Projected End Date- September/October.
Project Manager(s)- Daniel, Ajit.
Projected Planting Date- 5/6 or 5/11.
Crop(s)- Hemp: Grain and Fiber. cv. Grain/fiber (Carmagnola, Abound, & SS-Beta).
Irrigation - Solid set: over-head sprinklers.
Applications - Six (6) Nitrogen treatments, split between **four (4) applications.
Planting methods - 3' Mounds, planted in center 1.25'. Seeded by hand.
Data Collection & Sampling - Emergence, Stand count, Height, Flowering, Sex ratio, Seed hardening. TBD.
Project Maintenance - Weeding/cultivation between rows.
Equipment needed - Moldboard plow, Harrow disk, Row marker, others - TBD.
Field crew assistance - Yes. For irrigation and land preparation.

Land Request

Preferred block- Block 1.
Section- NW.
Area/Acres requested- Field size: L 260' x W 110'. (0.66 ac).
Greenhouse - N/A.

To Do: Update check list

Complete TREC Land Request
Complete TREC Greenhouse Request
Request faculty sponsor to complete UFarm request.

Land Preparation

Cultivation (mowing, moldboard plow, disc and level the field, form raised beds, drilling holes, etc.) - Moldboard plow, disc and level the field, form mounds.
Row Length - 25 ft.
In-Row Spacing - 4-6 inches.
Between-Row Spacing - 3'
Field Buffers - 60 ft.
Plot size - 12 ft wide (4 rows) x 25 ft long (300 ft2) x 18 trts x 4 reps = 216 ft wide x 130 ft long.
Seeding? (Direct, Broadcast, Incorporated, Drill, Earthway, etc.) - Direct seeding.
Transplants? - No.
Support Structure (Rebar, String, etc.) - NA.
Plastic Mulch? - NA.
Full image of field layout, design, and plot arrangement - Maps, layout and desins can be found on TEAMS.
Date to complete land preparation - 4/30-5/3.

To Do: Update check list

Complete TREC field preparation request
Row Crop
Plastic Culture
Fruit Crop

Irrigation

Drip Tape? (drip placement, emitter spacing, flow-rate GPM/100 ft., etc.) - NA.
Solid set? (overhead irrigation, microsprinkler) - Solid set, overhead.
Irrigation Timing (frequency and trigger) - As needed** [Once per week for first 2-3 weeks to germinate and establish**].
Irrigation Amount (per application) - 1 inch/ TBD.

To Do: Update check list

Complete TREC fieldwork request for irrigation.

Fertilizer Application

Label Name - Urea (N), Triple Super Phosphate (P), Sulfate of Potash (K)
Analysis rates (NPK, etc.) - 46-0-0 (N), 0-46-0 (P), 0-0-50 (K)
Application rate per acre -
Urea

```
 0 lbs N/ac 
```
```
 50 lbs N/ac 
```
```
 100 lbs N/ac 
```
```
 150 lbs N/ac 
```
```
 200 lbs N/ac 
```
```
 250 lbs N/ac.
```
TSP
```
 60 lbs P2O5/ac
```
SOP
```
 300 lbs K2O/ac
```

Application method (banded on top of the bed, soil incorporation, etc.) - Pre-plant incorporated, banded on top of the mound - except nitrogen.
Application timing and frequency - TBD.
Lab or field personnel - Daniel and Ajit.

To Do: Update check list

Fertilizer request form

Herbicide Application

Label Name - Dual Magnum.
Active ingredient(s) and concentration - S-metolachlor, benoxacor.
Resistance Management: Group 15 Herbicide.
Restricted Entry Interval (REI): 12 hours.
Application rate per acre - 1 pint.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - Post-planting, sprayer.
Application timing, frequency, and location - Once at post-planting. Applied across the entire plot (mounds and furrows).
Lab or field personnel - Daniel, Ajit, Jacob.

To Do: Update check list

Worker Protection Standard Training

Review "Training Workers and Handlers under the 2016 Revision Requirements" by Frederick M. Fishel.
Found in the TREC network Unit drive > TREC Pesticide Use information > WPS information

Pesticide request form

Project Maintenance Events

Research treatment application -
Weeding - TBD PRE: Dual Magnum.** POST: cultivation or hooded applications.
Scouting - Weekly.
Sampling -
Harvesting and Sampling -
Project removal-

To Do: Update check list

Equipment Request
Complete TREC fieldwork request well ahead of maintenance events.

Data Collection & Sampling

Database - TEAMS, GitHub.

Data type -

Rainfall, irrigation, temperature
Soil moisture
Mehlich 3 P, pre-plant, 0-6”, block-level
Total N (dry combustion), pre-plant, 0-6”, block-level
NO3- & NH4+, pre-plant, 0-6”, block-level
SOM, pre-plant, 0-6”, block-level
Soil water holding capacity
Plants/m2 after emergence
Leaf tissue nutrient concentrations (N & P) i. Stage 1 ii. Stage 2 iii. Stage 3 iv. Stage 4 v. Stage 5
```
Biomass at harvest 
```
```
Yield , moisture, test weight 
```

Mehlich 3 P, post-harvest, 0-6”, plot-level

Total N (dry combustion), post-harvest, 0-6”, plot-level

NO3- & NH4+, post-harvest, 0-6”, plot-level

```
SOM, post-harvest, 0-6”, plot-level.
```

Data storage -
Metadata -
Data collection -

-Protocols -

Timing -
Logistics -
Sample processing and analysis -

Contingencies

What important decisions are next to be made or remain a challenge?
What could possibly go wrong?

Add THC-testing data

The data from Erin in the DropBox should be formatted as .csv and uploaded to GitHub with information on sampling date and replication.

Remove extra embedded `hemp/` folder

Make sure the new data upload is contained in the root data/ folder.

Ping @Danielcalza

EDIS for hemp genetics

BiomassWeight_kg in GrainHarvest

These values in kg seem unreasonably large. Are they g? If so, update column name and metadata.

Reasons why hemp would flower on day ~56 26 June

-Autoflowering

-Instead of flowering at a specific day length, flowered as soon as days started getting shorter - I don't think this is happening - but, the younger hemp are not flowered yet

-Heat Stress from the last week - but, the younger hemp are not flowered yet

Two README.md?

Make sure the README file in the root directory is up-to-date and remove the file fromdata/.

Grain & Fiber Planting Date Trial - Project Preparation 2021 SOP

Project Objectives - To evaluate hemp (Cannabis sativa) varieties by four (4) planting dates.

Project Description - Early Planting Date Trial (4/26/2021) and (5/18/2021). Late Planting Date Trial (7/../2021) and (8/../2021).
Project Protocol - Two (2) Early and two (2) Late Planting Dates to be evaluated.
Field Design -
6 varieties x 4 reps x 2 dates. (20 trt).

Field size: L 75' x W 42'.

Plot size: L 10' x W 6'. (60 sqft).

*Total area: L 75' x W 168'. (0.56 ac).

Field Diagrams - Maps can be found on TEAMS.
Projected Start Date- Mid-April, Early-May. Mid-July, Early-August.
Projected End Date- September/October.
Project Manager(s)- Daniel, Ajit.
Projected Planting Date- April 26, 2021 (4/27/2021) and May 18, 2021 (5/18/2021). (7/../2021) and (8/../2021).
Crop(s)- Hemp (Cannabis sativa) Grain and Fiber. (Codimnono. Eletta. NWG 2730. Abound. SS-Beta. X-59).
Seeding rate: 200,000 live seeds/ac.
Irrigation - Solid set: over-head sprinklers.
Applications - Pre planting P and K. During trial N.
Planting methods - On mounds, by hand.
Data Collection & Sampling - Emergence, Stand count, Height, Flowering, Sex ratio, Seed hardening. TBD.
Project Maintenance - Weeding/cultivation between rows.
Equipment needed - Moldboard plow, Harrow disk, others - TBD.
Field crew assistance - Yes. For irrigation and land preparation.

Land Request

Preferred block- Block 1.
Section- SE and SW Corner.
Area/Acres requested- Total area: L 75' x W 84'. (0.14 ac). x 2 Early Planting Dates = (0.28 ac).
Greenhouse - NA.
To Do - Update check list

-[] Complete TREC Land Request

-[] Complete TREC Greenhouse Request

-[] Request faculty sponsor to complete UFarm request.

Land Preparation

Cultivation (mowing, moldboard plow, disc and level the field, form raised beds, drilling holes, etc.) - Moldboard plow, disc, and level the field, form mounds, bed press and cut seed furrows.
Field Length - 75'
Field width - 42'.
Plot width - 6'.
Plot length - 10'.
In-Row Spacing - TBD.
Between-Row Spacing - 3'.(36").
Field Buffers - 30-40'
Seeding - (Direct, Broadcast, Incorporated, Drill, Earthway, etc.) - Yes, Direct sown by hand.
Transplants - No.
Support Structure (Rebar, String, etc.) - NA.
Plastic Mulch - NA.
Full image of field layout, design, and plot arrangement - Yes, on TEAMS.
Date to complete land preparation - 4/23/2021 and 5/17/2021.
To Do - Update check list

-[] Complete TREC field preparation request

-[] Row Crop

Irrigation

Drip Tape - (drip placement, emitter spacing, flow-rate GPM/100 ft., etc.) - Solid set.
Solid set - (overhead irrigation, micro-sprinkler) - NA.
Irrigation Timing (frequency and trigger) - Once a week, if 1" of rainfall has not occurred already - TBD. Trigger - Soil moisture probes reading dry. TBD.
Irrigation Amount (per application) -1" - TBD.
To Do - Update check list

-[] Complete TREC field work request for irrigation.

Fertilizer Application

Label Name - SUPER FRUITER.
Analysis rates (NPK, etc.) -
Application rate per acre -
Application method (banded on top of the bed, soil incorporation, etc.) - Soil incorporation for the first application, banded on top of the mounds.
Application timing and frequency - Once, pre-planting.
Lab or field personnel - Ajit and Daniel.

-To Do - Update check list

-[] Fertilizer request form

Herbicide Application

Label Name - Dual Magnum.
Active ingredient(s) and concentration - S-metolachlor, benoxacor.
Resistance Management: Group 15 Herbicide.
Restricted Entry Interval (REI): 24 hours.
Application rate per acre - 1 pint.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - Post-planting, sprayer.
Application timing and frequency - Once at post-planting.
Lab or field personnel - Daniel, Ajit, Jacob.

To Do:

-[] Herbicide request form

Pesticide Application

Label Name - NA.
Active ingredient(s) and concentration - NA.
Application rate per acre - NA.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - NA.
Application timing and frequency - NA.
Lab or field personnel - NA.
To Do - Update check list

-[] Worker Protection Standard Training -

-[] Review "Training Workers and Handlers under the 2016 Revision Requirements" by Frederick M. Fishel.

-[] Found in the TREC network Unit drive > TREC Pesticide Use information > WPS information

-[] Pesticide request form

Project Maintenance Events

Research treatment application - Cultivars are the treatments to be evaluated.
Weeding - Hand cultivation.
Scouting - Weekly for volunteers outside of plots.
Sampling - tentative schedule found here: Sampling efforts schedule
Harvesting and Sampling -
Project removal -

To Do - Update check list

-[] Equipment Request -

-[] Complete TREC fieldwork request well ahead of maintenance events.

Data Collection & Sampling

Database - TEAMS, GitHub.
Data type - Paper, Digital.
Data storage - Paper, Excel. TEAMS, GitHub.
Metadata - TEAMS, GitHub.
Data collection - Weekly. Tentative schedule found here: Sampling efforts schedule
Protocols - Located on TEAMS.
Timing -
Logistics -
Sample processing and analysis -

Contingencies

What important decisions are next to be made or remain a challenge? - How/when to apply a pre-emergent herbicide.
What could possibly go wrong? - Equipment breakdowns, weather patterns, field prep delays, materials needed to be secured (seeds and fertilizer).

Height Table

Could you generate a table/figure of each variety to connect classification-Latitude, flowering time, and final height. Of each variety/planting date.

I want to make basic claims like plants that flowered in July were shorter than plants that flowered in August. Plants in May 1 planting were taller than later plantings. Very basic, not height curves.
thanks.

Will

Grain & Fiber P Study - Project Preparation 2021 SOP

Project Objectives - To determine EO hemp (Cannabis sativa) response to applied phosphorus.

Project Description - Grain & Fiber P Study
Project Protocol - 1 variety x 5 rates x 4 reps. (20 trt).

Field size: L 118' x W 84'. (0.23 ac).

Plot size: L 25' x W 12'. (300 sqft).

Field Design - RCB with 4 reps. Blocked by replication.
Field Diagrams - Maps can be found on TEAMS.
Projected Start Date- Mid-May. – 5/13-14.
Projected End Date- September/October.
Project Manager(s)- Ajit, Daniel.
Projected Planting Date- May 13-14th, 2021 (5/13/2021).
Crop(s)- Hemp: Grain & Fiber - cv.Carmagnola (Dual).
Irrigation - Drip-tape and possibly Solid set: over-head sprinklers.
Applications - Five (5) Phosphorus treatments, split between **four (4) applications.
Planting methods - Direct seeded into 3' Mounds, planted in center 1.25'.
Data Collection & Sampling -
Project Maintenance -
Equipment needed - Moldboard plow, Harrow disk, others - TBD.
Field crew assistance - Yes. For irrigation and land preparation.

Land Request

Preferred block- Block 1.
Section- NW corner.
Area/Acres requested- L 118' x W 84'. (0.23 ac).
Greenhouse - NA.

To Do: Update check list

-[] Complete TREC Land Request

-[] Complete TREC Greenhouse Request

-[] Request faculty sponsor to complete UFarm request.

Land Preparation

Cultivation (mowing, moldboard plow, disc and level the field, form raised beds, drilling holes, etc.) -
Row Length - 25'.
In-Row Spacing -
Between-Row Spacing - 3'.
Field Buffers - 40-60'.
Seeding? (Direct, Broadcast, Incorporated, Drill, Earthway, etc.) - Direct.
Transplants? - NA.
Support Structure (Rebar, String, etc.) - NA.
Plastic Mulch? - NA.
Full image of field layout, design, and plot arrangement - Layouts and designs can be found on TEAMS.
Date to complete land preparation - 5/12/2021.

To Do: Update check list

-[] Complete TREC field preparation request

-[] Row Crop

-[] Plastic Culture

-[] Fruit Crop

Irrigation

Drip Tape? (drip placement, emitter spacing, flow-rate GPM/100 ft., etc.) - NA.
Solid set? (overhead irrigation, microsprinkler) - Solid set - overhead.
Irrigation Timing (frequency and trigger) - As needed** [Once per week for first 2-3 weeks to germinate and establish**].
Irrigation Amount (per application) - 1 inch.

To Do: Update check list

-[] Complete TREC fieldwork request for irrigation.

Fertilizer Application

Label Name - Urea (N), Triple Super Phosphate (P), Sulfate of Potash (K)
Analysis rates (NPK, etc.) - 46-0-0 (N), 0-46-0 (P), 0-0-50 (K)
Application rate per acre -
Urea

```
 200 lbs/ac
```
TSP
```
 0 lbs P2O5/ac
```
```
 30 lbs P2O5/ac
```
```
 60 lbs P2O5/ac
```
```
 90 lbs P2O5/ac
```
```
 120 lbs P2O5/ac
```
SOP
```
 300 lbs K2O/ac
```

Application method (banded on top of the bed, soil incorporation, etc.) - Pre-plant incorporated - except phosphorus**?.
Application timing and frequency -
Lab or field personnel

To Do: Update check list

-[] Fertilizer request form

Herbicide Application

Label Name - Dual Magnum.
Active ingredient(s) and concentration - S-metolachlor, benoxacor.
Resistance Management: Group 15 Herbicide.
Restricted Entry Interval (REI): 12 hours.
Application rate per acre - 1 pint.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - Post-planting, sprayer.
Application timing and frequency - Once at post-planting.
Lab or field personnel - Daniel, Ajit, Jacob.

To Do:

-[] Herbicide request form

Pesticide Application

Label Name - NA.
Active ingredient(s) and concentration - NA.
Application rate per acre - NA.
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - NA.
Application timing and frequency - NA.
Lab or field personnel- NA.

To Do:

-[] Worker Protection Standard Training

Review "Training Workers and Handlers under the 2016 Revision Requirements" by Frederick M. Fishel.
Found in the TREC network Unit drive > TREC Pesticide Use information > WPS information

-[] Pesticide request form

Project Maintenance Events

Research treatment application -
Weeding -
Scouting -
Sampling -
Harvesting and Sampling -
Project removal-

To Do: Update check list

-[] Equipment Request

-[] Complete TREC field work request well ahead of maintenance events.

Data Collection & Sampling

Database - TEAMS, GitHub.
Data type -
Data storage -
Metadata -
Data collection
Protocols -
Timing -
Logistics
Sample processing and analysis
Protocols -
Timing -
Logistics -

Contingencies

What important decisions are next to be made or remain a challenge?
What could possibly go wrong?

Project Initiation Checklist - On-Farm Trials

Perigonal Bract Size

Now that we know that we large seed varieties, lets see if the area of the perigonal bracts is also larger before flowering or if there is no difference.

Design measurements for this

THC/CBD content in CBG hemp

This paper says it best. It's rooted in some other work by the same group.

Summary is - hemp homozygous for the B0 allele accumulates high CBG. It also accumulates low amounts of CBD and no detected amounts of THC. My guess is that it's a low efficiencies mutant of the CBDA gene. But it's great news that CBG HEMP MAY NEVER GO HOT if this pans out.

2005_paper_CBGhempfromB0allele.pdf

Day Length Figure

We should make a figure to represent day length differences by latitude.

I am attaching a csv with the number of 13.5 and 14.5 hour days per latitude. Also, is the excel sheet I used to calculate for each latitude.

How can we best process this data into a final figure?

Will

Add missing dry weights for grain harvest

Where does THC come from in Hemp?

Discussion and my notes of the origin of THC molecules in hemp plants.

Project Initiation Checklist - Grain & Fiber N study

What is the origin of the 0.3% THC distinction?

What information do we have to predict pollen drift?

Collect and submit soil and water samples for hemp field

On-Farm Trials - Project Preparation SOP

Project Information Overview
_
Project Objectives -
Project Description -
Project Protocol -
Field Design -
Field Diagrams -
Projected Start Date-
Projected End Date-
Project Manager(s)-
Projected Planting Date-
Crop(s)-
Irrigation -
Applications -
Planting methods -
Data Collection & Sampling -
Project Maintenance -
Equipment needed -
Field crew assistance -
_
Land Request
_
Preferred block-
Section-
Area/Acres requested-
Greenhouse -
_
To Do
Complete TREC Land Request
Complete TREC Greenhouse Request
Request faculty sponsor to complete UFarm request.
_
Land Preparation
_
Cultivation (mowing, moldboard plow, disc and level the field, form raised beds, drilling holes, etc.) -
Row Length -
In-Row Spacing
Between-Row Spacing -
Field Buffers -
Seeding? (Direct, Broadcast, Incorporated, Drill, Earthway, etc.) -
Transplants? -
Support Structure (Rebar, String, etc.) -
Plastic Mulch? -
Full image of field layout, design, and plot arrangement -
Date to complete land preparation -
_
To Do
Complete TREC field preparation request
Row Crop
Plastic Culture
Fruit Crop
_
Irrigation
_
Drip Tape? (drip placement, emitter spacing, flow-rate GPM/100 ft., etc.) -
Solid set? (overhead irrigation, microsprinkler) -
Irrigation Timing (frequency and trigger) -
Irrigation Amount (per application) -
_
To Do
Complete TREC field work request for irrigation.
_
Fertilizer Application
_
Label Name -
Analysis rates (NPK, etc.) -
Application rate per acre -
Application method (banded on top of the bed, soil incorporation, etc.) -
Application timing and frequency -
Lab or field personnel
_
To Do
Fertilizer request form
_
Pesticide Application
_
Label Name -
Active ingredient(s) and concentration -
Application rate per acre -
Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) -
Application timing and frequency -
Lab or field personnel
_
To Do
Worker Protection Standard Training
Review "Training Workers and Handlers under the 2016 Revision Requirements" by Frederick M. Fishel.
Found in the TREC network Unit drive > TREC Pesticide Use information > WPS information
Pesticide request form
_
Project Maintenance Events
_
Research treatment application -
Weeding -
Scouting -
Sampling -
Harvesting and Sampling -
Project removal-
_
To Do
Equipment Request
Complete TREC field work request well ahead of maintenance events.
_
Data Collection & Sampling
_
Database
Data type -
Data storage -
Metadata -
Data collection
Protocols -
Timing -
Logistics
Sample processing and analysis
Protocols -
Timing -
Logistics -
_
Contingencies
_
What important decisions are next to be made or remain a challenge?
What could possibly go wrong?

Add Important Project Documents to Dropbox Folder

Flowering data figure

@brymz

for the variety trial - could you order the varieties in columns according the their 'class' It would be nice to see a vertical column of all the early flowering, intermediate, cbd, and south Chinese. This would allow the reader to make and easy comparison between and within classes.

I would like the reader to see so identical the Canadian varieties were and importantly the difference between Puma-3 and Si-1.

This would also be a visual justification of the classes I assigned each group.

Do you think a red dot, or some signifier to point out where the 50% flowering time is?

Set up communication plan and decision tree for email/call-in filters

Emails should go to [email protected]
Graduate student / Extension agent team have first filter
- Zack - high-profile and/or direct relevance to research plan
- UFF - general relevance with clear intent for resource and/or funding support
- Site leader - farmer with enthusiasm | interest to volunteer | proximity to research location
- Roger - propagation | indoor production
- ??? - plant extracts | quality testing
- Direct to website - general request for information

Pollen drift

There is one paper that found cannabis pollen and weather patterns indicate hemp pollen found in Spain may be blowing in from Morocco.

They do a good job finding supporting information, but It's a very singular study. There have not been any further studies on this pollen transportation, and the Africa connection is a little speculative considering there must also be local marijuana grown in Spain.

There isn't data on how much pollen detected in the air might effect cultivation of females.

Planning and preparation of Hemp Invasion Risk Trial [June-October]

June
.
6/ - Land prep: Communicate with field crew for mowing, plowing, disking, and irrigation.
6/ - Measure to flag out area and units
6/ - Set up envelopes and weigh seeds
6/ - Secure fertilizer
6/ - Print all data sheets
.
July
.
7/10 - Fertilize and Planting
7/13-17 - Emergence data/ Damping-off
7/15 - PAR data
7/20 - Last emergence/ Damping-off
7/21 - Remove irrigation
.
August
.
8/6 - Mowing buffers.
8/7 - Height data
8/7 - Fertilize
8/13 - PAR data
.
September
.
9/4 - Height data
9/10 - PAR data
.
October
.
10/1 -PAR data
10/2 - Height data

10/23 - Termination

is there literature on humidity affecting hemp?

I did a literature search to double check. I found zero papers or decent sources describing the effects on humidity on hemp, but some remarks indicating the author felt 'high' humidity decreased resin. Nothing worth citing though.

Hemp Abandonment Trial Metadata

Block: Block composed of eight (8) sub-plots in blocks 1-4, and 9, and twenty (20) sub-plots in 5-8.
Row: Field rows numbered ascendingly from south to north.
Column: Field columns ordered alphabetically from east to west.
Group: Cluster of plants found post-management.
Height_cm: Height of plant at time of measurement in cm.
Total_Nmb_seed: Total number of seeds per plant.
`Total_seed_wgt: Total seed weight.
Wgt_of_10: Weight of ten (10) seeds.
Wgt_of_20: Weight of twenty (20) seeds.
M: Male
F: Female
V: Vegetative
WP: Walking path
PP: BLOCK 1-4
VT: BLOCK 5-9
PP2: BLOCK 10-13
PPP8: BLOCK 14-21

Florida Fiber industry 1895

I found a book about the Florida fiber industry written in 1895 (The Fibre Bearing Plants of Florida). They describe Agave sisalana as "Florida Hemp," the also refer to manila hemp (banana), invasive Urena lobata (congo jute), Bromelia sylvestris, pineapple, and Ramie in detail.

No where is cannabis mentioned, but all these fibers are referred to as hemp.

THis is additional evidence that there has never actually been a sativa production here for fiber

Add emails to Extension Team contacts list

The file /Hemp/Extension/ExtensionTeam.xlsx has a list of names of Extension agents that we are supposed to include in regular updates. The list needs emails added.

Tentative Land Prep schedule - Blks 1 & 10 [February-May]

February
.
2/25 - Disk. South section - No drag.
.
March
.
3/2 - Plow NW section.
3/11 - Disk NW section.
3/18 - Disk. South section w/drag.
3/23-31 - Leveling disks - as needed.
.
April
.
4/1-9 - Plant sorghum buffers.
4/5 – Second disking blocks 1 and 10.
4/19-4/26 – Third Final disking(as needed/weed control), row raking, prep and setting out irrigation for Planting Date Trial – Early (PDT-E) – Block 1 .
4/26 - Scratch rows. Planting of PDT-E-1.
4/26-5/3 – Third Final disking(as needed), bed shaping and drip install for EO-N Study – Block 10.
4/26-5/3 – Third Final disking(as needed), row raking and prep for Grain and Fiber N Study – Block 1.
.
May
.
5/3-5 - Planting.
5/6 – Planting of EO-N or Grain and Fiber N Study. **
5/4-7 - Lay out irrigation.
5/10 – Planting of EO-N or Grain and Fiber N Study. **
.
**Still undetermined which one will go in first.

Project Initiation Checklist - Essential Oil P Study

Answer legal questions about CBD and genetitcs

When does the transition to 2018 Farm Bill occur?
What is the role of DEA in the hemp program?
- Are there legal limitations on breeding and genetics experiments (e.g., CRISPR)?
- What is the procedure for international and domestic transportation?
Can we admit working with CBD plants? [FDACS - Yes]
Can we extract CBD as part of industry-driven research? [FDACS - Not yet]

Coordinates of 2019 plantings

PP1 and Variety trial
NE corner: 25.5106648, -80.5035612
NW corner: 25.5106533, -80.5037365
SE corner: 25.5100130, -80.5035226
SW corner: 25.5100191, -80.5037158
3rd planting
NE corner: 25.5109193, -80.5035418
NW corner: 25. 5108739, -80.5037446
SE conrner: 25.5108019, -80.5035491
SW corner: 25.518270,-80.5307704
4th planting
NE corner: 25.5103072, -80.5037050
NW corner: 25.5102963, -80.5038868
SE corner: 25.5100191, -80.5037158
SW corner: 25.5099958, -80.5039116

What is the current status of somatic and embryotic tissue culture?

Definte hemp material sampling for THC testing protocol

How will this be adapted to field testing mandated by FDACS?

Project Initiation Checklist - Invasion Risk Trial

Invasion Risk Trial - Project Preparation 2020 SOP

Hemp Invasion Risk Metadata

Sampling Dates

Start: 7/10/2020
End: 10/23/2020

Design and Methods

Fertilizer: [6-3-16].

50lb N/acre / 0.06 = 833.33 lbs fertilizer per acre.
833.33 / 4047 m2 = 0.206 lbs fertilizer per m2.
0.206 x 4 = 0.82 lbs of fertilizer per 4m2 plot.
0.82lb x 60 plots = ~50 lbs (49.2lbs).

Total 8 blocks [48 plots to be fertilizer]:

0.82lb x 48 plots = ~40 lbs (39.36lbs).
0.82lb = ~372g = ~360ml.

Varieties:

Han-NE
Helena
CFX-1

2020 viability (%).

43.3
12.3
33.0

For a 1000 viable seeds per plot.

2309 seeds
8130 seeds
3030 seeds

Seeds per plot (g).

Total seeds required + 20% contingency (kg).

Seed packets per variety:

Han-NE - 32
Helena - 32
CFX-1 - 32

Data_Products

Height_cm: Canopy height of each crop at a Sampling Month.
Damping-off: Number of plants dying, 0-3, [0: none. 1: Some. 2: Moderate. 3: Major].
Snail Damage:
Emergence: Count of emerging seedlings.
PAR: Photosynthetically active radiation.
Block: 1-8. Area containing 12 Plots.
Plot: 1-12. Area contained within Blocks.
Low-Nutrients: no nutrients will be added
High-Nutrients: represent an agricultural field under cultivation.
High-Disturbance: Full-tillage
`Low-Disturbance: No-tillage
Fibranova: Italy, Dual.
Si-1: South China, Grain.
CFX-1: Canada, Fiber.

Project Initiation Checklist - Grain & Fiber Planting Date Trial

Compile TREC IHPP

[] Assess application draft for section that are final, OR need more work.

which I need to ask Zack, send questions
Finalize sections that I can pull together.
follow up with Zack's answer
Assemble permit, send to Zack

Unless we are just on the temp threshold, the low emergence in later planting doesn't seem to be related to increase rain or soil temp recorded by the FAWN data.

on average, only a 4 degree difference in soil temp in 2 week period following each planting, but soil temp best explains decreased emergence - it actually rained more in the 2 weeks after May 1 than May 22.

Missing Height Data

PilotPlot1_summer_2019_PlantHeight.csv
PilotPlot2_summer_2019_PlantHeight.csv

Hemp Invasion Risk Metadata for TREC

Start: 7/10/2020
End: 10/23/2020
_
Feild layout:
Total area: 36 m x 48 m. (1,728 m2).
Eight (8) blocks - 22 m x 6 m. (132 m2).
Twelve (12) plots per block - 2m x 2m. (4 m2).
_
Irrigation:
Four (4) hours on the day of planting.
Rain on **
_
Fertilizer: [6-3-16].
50lb N/acre / 0.06 = 833.33 lbs fertilizer per acre.
833.33 / 4047 m2 = 0.206 lbs fertilizer per m2.
0.206 x 4 = 0.82 lbs of fertilizer per 4m2 plot.
_
0.82lb x 60 plots = ~50 lbs (49.2lbs).
_
Total 8 blocks - 48 of 96 plots to be fertilizer:
0.82lb x 48 plots = ~40 lbs (39.36lbs).
0.82lb = ~372g = ~360ml of fertilizer per 4m2 plot.
_
Varieties:

Han-NE
Helena
CFX-1
_
2020 viability (%).
43.3
12.3
33.0
_
For a 1000 viable seeds per plot.
2309 seeds
8130 seeds
3030 seeds
_
Seeds per plot (g).
62
150
58
_
Total seeds required + 20% contingency (kg).
3
7.2
8.4
_
Seed packets per variety:
Han-NE - 32
Helena - 32
CFX-1 - 32
_
Data_Products.
Height_cm: Canopy height of each crop at a Sampling Month.
Damping-off: Number of plants dying, 0-3, [0: none. 1: Some. 2: Moderate. 3: Major].
Snail Damage:
Emergence: Count of emerging seedlings.
PAR: Photosynthetically active radiation.
_
Block: 1-8. Area containing 12 Plots.
Plot: 1-12. Area contained within Blocks.
Low-Nutrients: no nutrients will be added
High-Nutrients: represent an agricultural field under cultivation.
High-Disturbance: Full-tillage
Low-Disturbance: No-tillage
Fibranova: Italy, Dual.
Si-1: South China, Grain.
CFX-1: Canada, Fiber.

Draft scaffolding of planting details

Upload variety trial data

Put them in the data/.

Flowering
Density
Height and Sex
Emergence

trec-agroecology / hemp Goto Github PK

hemp's Introduction

Hemp Metadata and Field Notes

hemp's People

Contributors

Stargazers

Watchers

Forkers

hemp's Issues

Project Objectives - To determine EO hemp (Cannabis sativa) response to applied nitrogen.

Land Request

Land Preparation

Irrigation

Fertilizer Application

Herbicide Application

Pesticide Application

Project Maintenance Events

Data Collection & Sampling

Contingencies

Project Objectives - To determine hemp (Cannabis sativa) response to applied nitrogen.

Land Request

Land Preparation

Irrigation

Fertilizer Application

Herbicide Application

Project Maintenance Events

Data Collection & Sampling

Contingencies

Project Objectives - To evaluate hemp (Cannabis sativa) varieties by four (4) planting dates.

Land Request

Land Preparation

Irrigation

Fertilizer Application

Herbicide Application

Pesticide Application

Project Maintenance Events

Data Collection & Sampling

Contingencies

Project Objectives - To determine EO hemp (Cannabis sativa) response to applied phosphorus.

Land Request

Land Preparation

Irrigation

Fertilizer Application

Herbicide Application

Pesticide Application

Project Maintenance Events

Data Collection & Sampling

Contingencies

Hemp Invasion Risk Metadata

Sampling Dates

Design and Methods

Varieties:

Data_Products

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