thejacksonlaboratory / cloudneo Goto Github PK
View Code? Open in Web Editor NEWCWL implementation of CloudNeo: A cloud pipeline for identifying patient-specific tumor neoantigens
License: Apache License 2.0
CWL implementation of CloudNeo: A cloud pipeline for identifying patient-specific tumor neoantigens
License: Apache License 2.0
I'm currently working on setting up CloudNeo for use with rabix, and I wanted to let you know that the installation instructions need updating.
For the netMHC and netMHCpan installations, the softwares should be downloaded to the main CloudNeo directory in order to use the recommended docker image build commands as specified in the wiki (which need a '-f' in front of the docker file paths, too).
This command within the Dockerfile for each of these tools was also giving me trouble:
RUN apt-get install -y python-biopython
but I was able to use
RUN wget https://bootstrap.pypa.io/get-pip.py
RUN python get-pip.py
RUN pip install biopython
instead, and it worked fine. Not sure if this is an issue on my end or a more general issue, though.
The polysolver build also requires a lot of files that aren't in the repository and must be tracked down (e.g. GATK v3.5 jar, mutect jar, etc.).
It's fairly doable to figure out these issues out on your own, but it would be good to have more accurate and thorough instructions in the wiki.
HI,
#The output of log:
[2018-11-15 10:59:53.710] [INFO] Job root.samtools_fastq has started
[2018-11-15 10:59:54.686] [INFO] Job root.Variant_Effect_Predictor has started
[2018-11-15 10:59:54.946] [ERROR] Job 3cf1bfc7-5fac-4f89-9d65-b9abaf9c11ac failed with exit code 2.
[2018-11-15 10:59:54.948] [INFO] Job 3cf1bfc7-5fac-4f89-9d65-b9abaf9c11ac failed with exit code 2.
[2018-11-15 10:59:55.669] [INFO] Failed to execute a Job
#I checked the error log
/bin/sh: 1: cannot create B10-B.sorted.rmdup.addhead.fastq: Permission denied
Thanks !
Baifeng
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