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Few general queries about filtar HOT 1 OPEN

ashokpatowary avatar ashokpatowary commented on August 17, 2024 1
Few general queries

from filtar.

Comments (1)

TBradley27 avatar TBradley27 commented on August 17, 2024

Hi @ashokpatowary,

Thank you very much for getting in touch about these issues. I am glad the tool is of interest to you.

I will try and address your message point-by-point:

  1. This is a good point you make, and this is a known limitation of FilTar. Currently FilTar only accepts Ensembl/Gencode formatted GTF files. This is an active area of development, and I look forward to updating you once this issue has been addressed. I have created a new issue (#8) specifically for this point.

  2. More accessible and extensive configuration options is also something that I am currently looking into. This is an issue that has partially been raised previously ( see #5). However, I now also see the need to include more comprehensive configuration options. Can you tell me please which transcript expression parameters you are most interested in please? As a short-term workaround, you could try editing the file 'FilTar/modules/quant_reads/salmon/quant_salmon.py' in order to specify parameter values directly in this wrapper script. However, I am hoping to implement a more user-friendly method of specifying these configuration options.

  3. At the moment, FilTar has only been tested with Illumina short-read data. However, integrating long-read data would be an exciting opportunity for the future. Could you specify which type of long-read data you are interested in please i.e. which sequencing technology/platform?

If you have any more comments or queries about the tool then do not hesitate to get in contact.

Many thanks,
Thomas

from filtar.

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