Comments (5)
Hello! Thank you again!
Barplots for the number of id. proteins per sample, and the percentage of missing values per samples are available in the QC tab, both are produced with the uploaded intensities before imputation:
In the Analysis parameters you can specify to only include proteins that have been identified in at least n replicates of the same group (e.g 2 out of 3 replicates), and you can also apply this filtering on valid values
filter on the bait samples only:
That being said, there are use cases were it would be beneficial to not perform the imputation at all.
This feature would interfere with the current functionality though, heatmaps (and to some degree profile plots) can not deal with missing values. I'll need to think about it, how to include this feature without breaking anything.
Best,
Sebastian
from amica.
Thanks for your quick response. I think it makes sense to keep the imputation. The bar plots for the protein groups is a wonderful way to show the summary. But maybe having additional tab that shows presence/absence for detected proteins across groups can add more useful information. something like this for proteins before imputation:
from amica.
I agree, this would be a good QC feature.
I will implement this.
Best,
Sebastian
from amica.
Thanks! Look forward to the new feature.
from amica.
Hello,
To keep this feature general for many samples, and because set comparisons become very difficult to visualize for many sets, I decided to opt for a heatmap.
You have 3 options to compare the overlap between samples in this heatmap (if you click on the wrench icons):
- Jaccard index (https://en.wikipedia.org/wiki/Jaccard_index)
- Overlap Coefficient (https://en.wikipedia.org/wiki/Overlap_coefficient)
- The number of shared proteins
In addition, you can inspect the values of these metrics in a data table.
These features are available in the "Protein Overlap" tab next to the Protein groups barplots.
Best,
Sebastian
from amica.
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from amica.