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Russel88 avatar Russel88 commented on August 26, 2024 1

CCTyper 1.6.2 now prints a GFF with all repeat and spacer coordinates

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Russel88 avatar Russel88 commented on August 26, 2024

Hey,

I'm glad you like the tool!
Extracting coordinates of all spacers is not that straightforward, unfortunately. The start and end in crisprs_all.tab refer to start and end of the entire CRISPR array.

If you run cctyper with --keep_tmp it will produce the raw output from minced in minced.out; from this file you can extract the positions of all repeats and spacers. The newest version of cctyper also detects CRISPR arrays by BLASTing repeats, and positions of these spacers is not readily obtainable. However, these arrays are somewhat rare (except if you're looking for IV-A3 CRISPRs specifically).

It would probably be a good idea to make cctyper output a gff with all the positions in a standardized way. I'll put that on my to do list.

I'm sorry that I can't provide an easy solution at the moment.

Best,
Jakob

from crisprcastyper.

genomesandMGEs avatar genomesandMGEs commented on August 26, 2024

Thanks for the detailed explanation Russel!

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