q2 picrust2 Tutorial error about picrust2

[dong1984]

Hi gavinmdouglas, Thanks very much for your help. The problem is that I run out of the computational resources. By giving more computational resources, I have successfully pass the fragmentation insertion step!

[gavinmdouglas]

What version of QIIME2 are you using?

[dong1984]

qiime2-2018.11
fragment-insertion 4.3.5
q2-fragment-insertion 2018.11.0

[gavinmdouglas]

Is anything written to /tmp/qiime2-q2cli-err-cby4nvz1.log?

I would first recommend re-downloading the files in case they are malformed. You could then try re-installing qiime2-2018.11 (which includes q2-fragment-insertion).

[dong1984]

I failed to find the /tmp/qiime2-q2cli-err-cby4nvz1.log
I re-downloaded the files and also updated qiime2 to qiime2-2019.1, but same error there...

[gavinmdouglas]

That's strange - those exact commands have been run numerous times in the qiime2-2018.11 defined environment so I'm not sure why SEPP would fail. You can export the input files (with qiime tools export) and run the run-sepp.sh command yourself to see a clearer error message though. The -a and -t options specify the reference files and the first input option would be the mammal study sequences.