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d4straub avatar d4straub commented on June 15, 2024 1

Yes, I just checked: Unicycler assembly (used for hybrid assembly) includes indeed already polishing with Pilon. So no additional polishing step is needed.
I close that issue, open another one if you recognize other oddities!

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d4straub avatar d4straub commented on June 15, 2024

My notes say that assembly polishing is already done by Unicycler, that is used for hybrid assembly. Do you have there an opposing example/guideline/source? I am open to changing that, but need some sort of reference that this is beneficial.

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varunshamanna avatar varunshamanna commented on June 15, 2024

I am kind of new to nanopore analysis. I think from your code it means that assembly polishing is not required when doing the hybrid assembly. Is that right? Correct me if I am wrong. If it is so please close the issue.

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