Comments (4)
For ion preprocessing the forward primer is required, along with a valid barcode sequence. The reverse primer is removed if it is found. https://amptk.readthedocs.io/en/latest/pre-processing.html
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If not found, the read is maintained or is dropped from the processing?
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Reads without valid barcodes or forward primers are discarded in the ion workflow. Not sure what isnβt clear in the docs?
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I think is clear now. I didn't understand that only forward primer is necessary for the processing. I thought that only the read would pass the processing if both primers forward and reverse were present in the read.
That helps a lot, thank you Jon!
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