Requested array size exceeds VM limit about abra

[mozack]

Could you please post the output of:

wc -l /scratch/TCGA-DD-A1EA-01A-11D-A12Z-10_Illumina_1410534136/clean_contigs.fasta
and
ls -lh /scratch/TCGA-DD-A1EA-01A-11D-A12Z-10_Illumina_1410534136/

[joonlee3]

I used your commands for another failed job. Below are what I've got from
the commands. If you have any insights/suggestions, please let me know.

Thanks,
Joon

wc -l
/scratch/TCGA-BC-A216-01A-11D-A152-10_Illumina_TCGA-BC-A216-11A-11D-A152-10_Illumina_1410987440/clean_contigs.fasta

24965046
/scratch/TCGA-BC-A216-01A-11D-A152-10_Illumina_TCGA-BC-A216-11A-11D-A152-10_Illumina_1410987440/clean_contigs.fasta

ls -lh
/scratch/TCGA-BC-A216-01A-11D-A152-10_Illumina_TCGA-BC-A216-11A-11D-A152-10_Illumina_1410987440/

*drwxrwxrwx 3 ................ 4.0K Sep 17 23:38 sorttmp/ *

*drwxr-xr-x 2 ................ 4.0K Sep 18 06:14 temp1/ *

*drwxr-xr-x 2 ................ 4.0K Sep 18 06:14 temp2/ *

*drwxr-xr-x 2 ................ 4.0K Sep 17 16:57 unaligned/ *

-rw-r--r-- 1 ................ 22 Sep 18 03:21 clean_contigs.fasta.amb

-rw-r--r-- 1 ................ 2.0G Sep 18 03:21 clean_contigs.fasta.ann

-rw-r--r-- 1 ................ 2.2M Sep 17 23:38
all_contigs_chim_sorted.bai

-rw-r--r-- 1 ................ 232M Sep 17 23:32 all_contigs_chim.bam

-rw-r--r-- 1 ................ 310M Sep 18 00:07
all_contigs_chim_chopped.bam

-rw-r--r-- 1 ................ 234M Sep 17 23:38
all_contigs_chim_sorted.bam

-rw-r--r-- 1 ................ 4.6G Sep 18 03:18 clean_contigs.fasta.bwt

-rw-r--r-- 1 ................ 5.2G Sep 17 18:55 all_contigs.fasta

-rw-r--r-- 1 ................ 6.3G Sep 18 00:11 clean_contigs.fasta

-rw-r--r-- 1 ................ 0 Sep 17 17:05 sv_contigs.fasta

-rw-r--r-- 1 ................ 1.2G Sep 18 03:21 clean_contigs.fasta.pac

-rw-r--r-- 1 ................ 2.3G Sep 18 03:34 clean_contigs.fasta.sa
http://clean_contigs.fasta.sa

-rw-r--r-- 1 ................ 6.3G Sep 17 19:36 all_contigs_in_region.sam

-rw-r--r-- 1 ................ 6.3G Sep 17 19:32 all_contigs.sam

*-rw-r--r-- 1 ................ 900K Sep 17 16:57 libAbra.so *

On Mon, Sep 15, 2014 at 4:34 PM, Lisle Mose [email protected]
wrote:

Could you please post the output of:

wc -l
/scratch/TCGA-DD-A1EA-01A-11D-A12Z-10_Illumina_1410534136/clean_contigs.fasta
and
ls -lh /scratch/TCGA-DD-A1EA-01A-11D-A12Z-10_Illumina_1410534136/

—
Reply to this email directly or view it on GitHub
#11 (comment).

Delight yourself in the LORD and he will give you the desires of your
heart.
Commit your way to the LORD; trust in him and he will do this:
He will make your righteousness shine like the dawn, the justice of your
cause like the noonday sun.
(Psalms 37:4 - 6)

[mozack]

I suspect this particular sample may not have failed in the same way.

The original problem appears to be due to too many contigs assembled. You may need to experiment with more aggressive graph pruning settings (or try to identify and exclude subregions that are particularly noisy). See --mbq and --mnf

For the second case, could I trouble you to compress and send the log file to lmose at unc dot edu ?

[mozack]

Just to clarify, more RAM won't correct that first problem.