Default of ROI background correction about imagej.github.io

[ctrueden]

@maurosilber wrote:

I tried looking into the code, but could not found it.

If you are using Fiji, you can find the source code by typing "Subtract Background" into the search bar, then clicking the "Source" button in the right-hand pane. Here is the code:

https://github.com/imagej/ImageJA/blob/v1.53k/src/main/java/ij/plugin/filter/BackgroundSubtracter.java

Personally, I do not like the "Subtract Background" command; the algorithm seems to have weird artifacts to me. But I never dug into the code to find out why. I just use a workflow like this instead:

1. Duplicate image
2. Gaussian blur the duplicate with large radius
3. Subtract the blurred image from the original

Please feel warmly welcome to improve the Image Intensity Processing page, and really any page, on the wiki—that's why it is a wiki. The original authors of those Scientific Imaging Tutorials pages are mostly long gone, so it's up to the community to improve them as ImageJ evolves.