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wolski avatar wolski commented on May 28, 2024

Dear Craig,

Thank you for using prolfqua and reporting the issue with the filtering. The commit should fix it:
926e041

Regarding your point 2:
See also this issue on the DiaNN GitHub repository:
vdemichev/DiaNN#398

I agreed with Vadim's opinion that:
"But I don't think this journal request makes sense for most analyses, so to formally comply with it can also calculate the number of stripped sequences matching the protein before the filtering."

And therefore I filter first for number of peptides and then filter for Q-values.

regards
W

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wolski avatar wolski commented on May 28, 2024

I am closing the issue. If you have any more questions, please add them here, and we can reopen the issue.

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clawless-inoviv avatar clawless-inoviv commented on May 28, 2024

Thank you for this and the link to the explanation.

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