Comments (3)
Dear Craig,
Thank you for using prolfqua and reporting the issue with the filtering. The commit should fix it:
926e041
Regarding your point 2:
See also this issue on the DiaNN GitHub repository:
vdemichev/DiaNN#398
I agreed with Vadim's opinion that:
"But I don't think this journal request makes sense for most analyses, so to formally comply with it can also calculate the number of stripped sequences matching the protein before the filtering."
And therefore I filter first for number of peptides and then filter for Q-values.
regards
W
from prolfqua.
I am closing the issue. If you have any more questions, please add them here, and we can reopen the issue.
from prolfqua.
Thank you for this and the link to the explanation.
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