Comments (3)
Hey Cameron,
Which results are you after that require all genomes to be processed at once? I always imagine people just running new genomes separately. The CheckM results for a given genome are independent of the other genomes being processed.
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Hi,
It's completely a convenience function aimed at being able to update the output files so that I could run the qa and plotting commands on the whole dataset to get complete summary files.
I figured CheckM would look in the storage directory and read in already processed files in order to generate the plots/summary output. And I figured these storage files are generated fresh each time I run CheckM such that the most recent one overwrites older ones. I'm basing this off the .tsv files in the storage directory. I can't tell if information is being calculated from them or if everything is being done from scratch for each genome when I run summary commands and then the concatenated result is being given to me.
Anyway, I imagined that if I ran CheckM on 100 genomes and then later ran CheckM again using the same output directories on one more genome, that the output from the single genome would overwrite the output from the 100 and I would effectively lose the analysis on them. Is this the case or does CheckM read/store the data in such a way that running an analysis like that would give me 101 genomes (what I want it to do) instead of 1?
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No, it will not update the processed genomes so you have results for 101. Actually, the lineage_wf command will refuse to run if you provide it with an existing directory so previous results are not overwritten.
I agree some sort of update command would be useful, but this is unlikely to happen in the short term. I suggest just processing new genomes to a new output directory. This wouldn't help with generating plots, but it is easy enough to concatenate tables together.
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Related Issues (20)
- Is there a possibility to have the distance between the putative genome and the closest reference genome? HOT 1
- FileNotFoundError for concatenated.tre file? HOT 1
- Unexpected error: <class 'RecursionError'> HOT 2
- Placing 3 bins into the genome tree with pplacer Killed HOT 4
- AttributeError: 'Namespace' when running 'checkm coverage' HOT 1
- bin_qa_plot
- Customizing CheckM Dataset & Gene Marker Selection for a Species
- Reference data is missing HOT 1
- don't find Strain heterogeneity info
- FileNotFoundError: [Errno 2] No such file or directory: 'checkm_folder/storage/tree/concatenated.tre' HOT 8
- IndexError: list index out of range HOT 1
- Understanding contamination value HOT 2
- ERROR conda.core.link:_execute(952): An error occurred while installing package 'bioconda::checkm-genome-1.2.2-pyhdfd78af_1'. HOT 1
- Unexpected error: <class 'KeyError'>
- FileNotFoundError: [Errno 2] No such file or directory: '/home/majorram/anaconda3/envs/checkm/hmms/phylo.hmm' HOT 3
- Chekm test: line 1: 16491 Bus error (core dumped) pplacer -j 1 HOT 2
- maximum number of genomes/MAGs to run checkm HOT 2
- WARNING: Expected all files to contain sequences in amino acid space. HOT 1
- local variable 'seqId' referenced before assignment
- 'utf-8' codec can't decode byte 0xb0 in position 37: invalid start byte HOT 1
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