Comments (4)
Sorry for not responding earlier, @stefcamp, I was on parental leave and didn't see your issue earlier.
CAMITAX cannot find or access the CheckM reference data. Did you run nextflow run CAMI-challenge/CAMITAX/init.nf --db /path/to/db/folder
prior to executing CAMITAX to download the reference data? Else please let me know whether you run CAMITAX with -profile docker
to make use of BioContainers or installed all dependencies manually, and we'll take it from there.
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Hi I keep having this same issue. I thought it was an installation issue (even though it worked fine previously), so I installed on a new virtual machine. I am still getting the same error though with a new installation.
I ran:
nextflow run CAMI-challenge/CAMITAX -profile docker --db ../../camitax_data/ --i ./ --x fasta
And got this error:
Error executing process > 'checkm (K_1000.001)'
Caused by:
Process checkm (K_1000.001)
terminated with an error exit status (126)
Command executed:
Set CheckM root data location
echo camitax_data/checkm/ | checkm data setRoot camitax_data/checkm/
Extract 16S rRNA gene sequences with Nhmmer
checkm ssu_finder -t 8 -x fasta K_1000.001.fasta . ssu_finder 2>&1
ln -s ssu_finder/ssu.fna K_1000.001.ssu.fna
Phylogenetic placement onto reduced reference tree
checkm lineage_wf -t 8 --reduced_tree -x fasta . checkm
checkm qa -o 2 --tab_table checkm/lineage.ms checkm > K_1000.001.checkm.tsv
Command exit status:
126
Command output:
(empty)
Command error:
docker: Got permission denied while trying to connect to the Docker daemon socket at unix:///var/run/docker.sock: Post http://%2Fvar%2Frun%2Fdocker.sock/v1.39/containers/create?name=nxf-IA0bM3ooRxKasszrIFcvWZWq: dial unix /var/run/docker.sock: connect: permission denied.
See 'docker run --help'.
Work dir:
/home/ubuntu/camitax_analyses/sharon/work/2d/f08fdbc10808d640d5e3e4ca7368d4
Tip: view the complete command output by changing to the process work dir and entering the command cat .command.out
Please help!
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I think I have soved this issue - an problem with the docker configuration and permissions on my server. Camitax is now running, so hopefully no more errors!
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I'm glad you solved the problem, unfortunately docker sometimes has problems with permissions.
If anything comes up, feel free to reopen
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Related Issues (20)
- Review final taxID assignments HOT 1
- State if 16S was found
- Confidence values HOT 3
- Scientific name HOT 1
- warning process name deprecated HOT 7
- Check for Nextflow version HOT 1
- Update CAMITAX db HOT 3
- Installation and running HOT 8
- start with conda HOT 2
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- Suggestion : Run checkm on more than 1 MAG at a time HOT 1
- Issues installing docker HOT 1
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- not working with singularity HOT 1
- Assembly statistics
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