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paulamartingonzalez avatar paulamartingonzalez commented on June 2, 2024

Dear Votti, I have been trying to run the pipeline on my own IMC images (I have them already in tiff version) and I am receiving the following error in CellProfiler preprocessing pipeline:

"The pipeline cannot be started because of a configuration problem:The pipeline did not identify any image sets. Please correct any problems in your input module settings and try again."

I added the files to the file list already. It is working in your test data. Do you have any suggestions of what is missing? Thx in advance

from imcsegmentationpipeline.

votti avatar votti commented on June 2, 2024

Hi!
How did you convert them to .tiff?
The Cellprofiler pipelines rely on the standardized naming that comes out from preprocessing the tiff files using these scripts.
If you use a custom naming, you need to adapt the way cellprofiler identifies images, namely the Metadata section and/or the NamesAndTypes section.

CellProfiler preprocessing pipeline: which step do you mean? 1_prepare_ilastik? There one would need to change how image stacks to be used for ilastik are identified. -> Change the Select the rule criteria section.

from imcsegmentationpipeline.

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