Deseq error about astrobiomike.github.io

[XELong]

Yes!!! The tutorial had remove the first four column for further analysis by the following step!
count_tab <- read.table("ASVs_counts-no-contam.tsv", header=T, row.names=1,
check.names=F, sep="\t")[ , -c(1:4)]

[AstrobioMike]

Hi there, Long,

It seems like the rows in the sample info table are not matching up with the columns in the count table. I don't know where that would have happened in your processing. The preview images of each looks like one of them starts with S01 and the other starts with S05. So maybe you dropped some from the count table? There is code in the tutorial that drops 4 "blanks" I think, maybe you did something like that. You just have to find where you dropped those samples. You have to explore the tables more closely. Use things like dim(sample_info_table) and row.names(sample_info_table), and compare that to what you get from dim(count_tab) and col.names(count_tab).

Check those all throughout your R code from the start and find where they diverge.

Good luck!